López Gustavo E, Peña Sabrina, Escobar Gabriela I, Hasan Déborah B, Lucero Nidia E
Agricultural Sciences Faculty, Lomas de Zamora National University, Ruta 4 Km 2.5, 1836 Llavallol, Buenos Aires, Argentina.
Agricultural Sciences Faculty, Lomas de Zamora National University, Ruta 4 Km 2.5, 1836 Llavallol, Buenos Aires, Argentina.
Rev Argent Microbiol. 2018 Jul-Sep;50(3):285-289. doi: 10.1016/j.ram.2017.08.004. Epub 2018 Jan 5.
Ovine cattle was introduced into America during the Spanish conquest with the second journey of Columbus to the Antilles and was disseminated throughout the region. In 1587, sheep were introduced into Argentina, later developing into the "Creole" breed. We selected 486 animals from different Argentine provinces with the aim of determining the serological status of brucellosis caused by Brucella melitensis and Brucella ovis. For the detection of antibodies against smooth Brucella spp., the Rose Bengal test (RBT) was performed as screening test while the serum agglutination test (SAT) and 2 mercapto-ethanol (2ME) were run as a confirmatory technique. Moreover, for the detection of antibodies against rough Brucella spp., we used the rapid slide agglutination test (RSAT) for screening and an indirect ELISA (IELISA) as confirmatory assay. This study showed that the total positive percentage of brucellosis due to B. ovis was 2.9%. Excluding the animals mixed with the Suffolk breed; seropositivity would be 0.6%. All animals tested negative for brucellosis caused by B. melitensis.
在西班牙征服时期，随着哥伦布第二次前往安的列斯群岛的航行，绵羊被引入美洲，并在该地区传播开来。1587年，绵羊被引入阿根廷，后来发展成为“克里奥尔”品种。我们从阿根廷不同省份挑选了486只动物，目的是确定由羊布鲁氏菌和绵羊布鲁氏菌引起的布鲁氏菌病的血清学状况。为检测针对光滑型布鲁氏菌属的抗体，采用玫瑰红试验（RBT）作为筛查试验，同时采用血清凝集试验（SAT）和2-巯基乙醇（2ME）作为确证技术。此外，为检测针对粗糙型布鲁氏菌属的抗体，我们采用快速玻片凝集试验（RSAT）进行筛查，并用间接酶联免疫吸附测定（IELISA）作为确证试验。本研究表明，绵羊布鲁氏菌引起的布鲁氏菌病总阳性率为2.9%。排除与萨福克品种杂交的动物后，血清阳性率为0.6%。所有动物对羊种布鲁氏菌引起的布鲁氏菌病检测均为阴性。
