Masuda Natsumi, Mantani Youhei, Yuasa Hideto, Yoshitomi Chiaki, Arai Masaya, Nishida Miho, Qi Wang-Mei, Kawano Junichi, Yokoyama Toshifumi, Hoshi Nobuhiko, Kitagawa Hiroshi
Laboratory of Histophysiology, Department of Bioresource Science, Graduate School of Agricultural Science, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe, Hyogo 657-8501, Japan.
Department of Basic Veterinary Medicine, College of Veterinary Medicine, Inner Mongolia Agricultural University, 306 Zhaowuda Road, Huhhot, Inner Mongolia 010018, P. R. China.
J Vet Med Sci. 2018 Mar 24;80(3):395-404. doi: 10.1292/jvms.17-0686. Epub 2018 Jan 1.
The distributions of β-defensin 1 and 2 in secretory host defense system throughout respiratory tract of healthy rats were immunohistochemically investigated. In the nasal epithelium, a large number of non-ciliated and non-microvillous cells (NCs) were immunopositive for both β-defensin 1 and 2, whereas a small number of goblet cells (GCs) were immunopositive only for β-defensin 1. Beta-defensin 2-immunopositive GCs were few. In the nasal glands, a small number of acinar cells and a large number of ductal epithelial cells were immunopositive for both β-defensins. In the laryngeal and tracheal epithelia, a very few NCs and GCs were immunopositive for both β-defensins. In laryngeal and tracheal glands, a very few acinar cells and a large number of ductal epithelial cells were immunopositive for both β-defensins. In the extra-pulmonary bronchus, a small number of NCs were immunopositive for both β-defensins. A small number of GCs were immunopositive for β-defensin 1, whereas few GCs were immunopositive for β-defensin 2. From the intra-pulmonary bronchus to alveoli, a very few or no epithelial cells were immunopositive for both β-defensins. In the mucus and periciliary layers, β-defensin 1 was detected from the nose to the extra-pulmonary bronchus, whereas β-defensin 2 was weakly detected only in the nose and the larynx. These findings suggest that the secretory sources of β-defensin 1 and 2 are mainly distributed in the nasal mucosa and gradually decrease toward the caudal airway in healthy rats.
采用免疫组织化学方法研究了健康大鼠呼吸道分泌性宿主防御系统中β-防御素1和2的分布。在鼻上皮中，大量无纤毛和无微绒毛细胞（NCs）对β-防御素1和2均呈免疫阳性，而少数杯状细胞（GCs）仅对β-防御素1呈免疫阳性。β-防御素2免疫阳性的杯状细胞很少。在鼻腺中，少数腺泡细胞和大量导管上皮细胞对两种β-防御素均呈免疫阳性。在喉和气管上皮中，极少数无纤毛细胞和杯状细胞对两种β-防御素均呈免疫阳性。在喉和气管腺中，极少数腺泡细胞和大量导管上皮细胞对两种β-防御素均呈免疫阳性。在肺外支气管中，少数无纤毛细胞对两种β-防御素均呈免疫阳性。少数杯状细胞对β-防御素1呈免疫阳性，而对β-防御素2呈免疫阳性的杯状细胞很少。从肺内支气管到肺泡，极少或没有上皮细胞对两种β-防御素均呈免疫阳性。在黏液层和纤毛周围层，从鼻腔到肺外支气管均可检测到β-防御素1，而仅在鼻腔和喉部可微弱检测到β-防御素2。这些结果表明，在健康大鼠中，β-防御素1和2的分泌来源主要分布于鼻黏膜，并向尾端气道逐渐减少。
