Forman L J, Marquis D E, Stevens R, Adler R, Vasilenko P
Diabetes. 1985 Nov;34(11):1104-7. doi: 10.2337/diab.34.11.1104.
Immunoreactive beta-endorphin (IR-BE) was measured by radioimmunoassay in the anterior pituitary (AP), neurointermediate lobe of the pituitary (NIL), and hypothalamus of female rats 4 wk after being made diabetic by a single injection of streptozocin (STZ). STZ-induced diabetes resulted in a significant reduction in the content and concentration of IR-BE in the AP and the content of IR-BE in the hypothalamus. Total hypothalamic protein was also significantly diminished. IR-BE levels in the NIL were unchanged. Column chromatography indicated that the reduction in IR-BE in the AP of the diabetic female rats represented a decrease in peptides that co-eluted with beta-endorphin and beta-lipotropin. In the hypothalamus, the reduction in IR-BE was represented solely by a decrease in a peptide co-eluting with beta-endorphin. Beta-lipotropin was not detectable in the hypothalami of control or diabetic female rats. These results suggest that, in the rat, diabetes may produce alterations in the mechanism(s) that regulate endogenous opiate levels in the pituitary and hypothalamus.
通过放射免疫分析法测定了单次注射链脲佐菌素（STZ）致糖尿病4周后的雌性大鼠垂体前叶（AP）、垂体神经中间叶（NIL）和下丘脑的免疫反应性β-内啡肽（IR-BE）。STZ诱导的糖尿病导致AP中IR-BE的含量和浓度以及下丘脑IR-BE的含量显著降低。下丘脑总蛋白也显著减少。NIL中的IR-BE水平未发生变化。柱色谱分析表明，糖尿病雌性大鼠AP中IR-BE的减少表现为与β-内啡肽和β-促脂素共洗脱的肽减少。在下丘脑中，IR-BE的减少仅表现为与β-内啡肽共洗脱的肽减少。在对照或糖尿病雌性大鼠的下丘脑中未检测到β-促脂素。这些结果表明，在大鼠中，糖尿病可能会改变调节垂体和下丘脑内源性阿片水平的机制。
