Zhang Yu, Sun Rui, Zhou Aijuan, Zhang Jiaguang, Luan Yunbo, Jia Jianna, Yue Xiuping, Zhang Jie
State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin, 150090, China.
College of Environmental Science and Engineering, Taiyuan University of Technology, Taiyuan, 030024, China.
AMB Express. 2018 Jan 8;8(1):2. doi: 10.1186/s13568-017-0534-7.
Most studies have employed aeration-biofiltration process for the simultaneous removal of iron, manganese and ammonia in groundwater. However, what's inside the "black box", i.e., the potential contribution of functional microorganisms behavior and interactions have seldom been investigated. Moreover, little attention has been paid to the correlations between environmental variables and functional microorganisms. In this study, the performance of industrial-scale biofilters for the contaminated groundwater treatment was studied. The effluent were all far below the permitted concentration level in the current drinking water standard. Pyrosequencing illustrated that shifts in microbial community structure were observed in the microbial samples from different depths of filter. Microbial networks showed that the microbial community structure in the middle- and deep-layer samples was similar, in which a wide range of manganese-oxidizing bacteria was identified. By contrast, canonical correlation analysis showed that the bacteria capable of ammonia-oxidizing and nitrification was enriched in the upper-layer, i.e., Propionibacterium, Nitrosomonas, Nitrosomonas and Candidatus Nitrotoga. The stable biofilm on the biofilter media, created by certain microorganisms from the groundwater microflora, played a crucial role in the simultaneous removal of the three pollutants.
大多数研究采用曝气生物过滤工艺同时去除地下水中的铁、锰和氨。然而，“黑匣子”内部的情况，即功能微生物行为和相互作用的潜在贡献很少得到研究。此外，环境变量与功能微生物之间的相关性也很少受到关注。在本研究中，对用于处理受污染地下水的工业规模生物滤池的性能进行了研究。出水均远低于现行饮用水标准规定的允许浓度水平。焦磷酸测序表明，在滤池不同深度的微生物样本中观察到了微生物群落结构的变化。微生物网络显示，中层和深层样本中的微生物群落结构相似，其中鉴定出了多种锰氧化细菌。相比之下，典型相关分析表明，能够进行氨氧化和硝化的细菌在上层富集，即丙酸杆菌、亚硝化单胞菌、亚硝化单胞菌和硝化刺菌属。由地下水中的某些微生物形成的生物滤池介质上的稳定生物膜，在同时去除这三种污染物方面发挥了关键作用。
