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采用选定离子流管-质谱法监测由起始培养物引发的液态小麦和埃塞俄比亚画眉草酸面团发酵。

Monitoring of starter culture-initiated liquid wheat and teff sourdough fermentations by selected ion flow tube-mass spectrometry.

机构信息

Research Group of Industrial Microbiology and Food Biotechnology, Vrije Universiteit Brussel, Brussels, Belgium.

出版信息

J Sci Food Agric. 2018 Jul;98(9):3501-3512. doi: 10.1002/jsfa.8869. Epub 2018 Feb 20.

Abstract

BACKGROUND

Selected ion flow tube-mass spectrometry (SIFT-MS) is a direct-injection mass spectrometric technique that has been introduced recently into the field of food and flavor analysis. It also shows potential for use in the monitoring of food fermentations. Therefore, this study aimed at the online monitoring of different volatile compounds produced during starter culture-initiated liquid sourdough fermentations by SIFT-MS, for which a new workflow was developed.

RESULTS

The online monitoring of the volatile sample compounds acetoin and ethyl acetate, diacetyl, and ethanol was made possible during the production of sourdoughs obtained through fermentation with several interesting strains belonging to the species Lactobacillus crustorum, Lactobacillus fermentum, Lactobacillus hilgardii, Lactobacillus nagelii, Lactobacillus sakei, and Gluconobacter oxydans. Acetoin and ethyl acetate could not be distinguished based solely on SIFT-MS data. Diacetyl production was monitored in the case of Lb. crustorum LMG 23699 as a starter culture strain, thereby making the distinction between those volatiles produced in sourdough without extra ingredients added or after the addition of citrate or malate.

CONCLUSION

Starter culture-initiated liquid sourdough fermentations were monitored successfully. The volatile compound production of the different starter culture strains tested reflected differences in their metabolism and/or competitiveness in a sourdough matrix. © 2018 Society of Chemical Industry.

摘要

背景

选择离子淌度质谱(SIFT-MS)是一种直接进样质谱技术,最近已被引入食品和风味分析领域。它在食品发酵监测方面也显示出应用潜力。因此,本研究旨在通过 SIFT-MS 在线监测启动培养引发的液体酸面团发酵过程中产生的不同挥发性化合物,为此开发了一种新的工作流程。

结果

通过发酵几种有趣的菌株,包括 Lactobacillus crustorum、Lactobacillus fermentum、Lactobacillus hilgardii、Lactobacillus nagelii、Lactobacillus sakei 和 Gluconobacter oxydans,实现了在线监测酸面团生产过程中挥发性样品化合物乙酰基和乙酸乙酯、双乙酰和乙醇的监测。仅根据 SIFT-MS 数据无法区分乙酰基和乙酸乙酯。监测到 Lb. crustorum LMG 23699 作为启动培养菌株时双乙酰的产生,从而区分了在没有添加额外成分的酸面团中和添加柠檬酸盐或苹果酸盐后的挥发性物质的产生。

结论

成功监测了启动培养引发的液体酸面团发酵。测试的不同启动培养菌株的挥发性化合物产生反映了它们在酸面团基质中的代谢和/或竞争力的差异。 © 2018 化学工业协会。

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