胰岛冷冻保存的系统评价
Systematic review of islet cryopreservation.
作者信息
Kojayan Greg G, Alexander Michael, Imagawa David K, Lakey Jonathan R T
机构信息
a Department of Surgery , University of California Irvine , Orange , CA , USA.
b Department of Biomedical Engineering , University of California Irvine , Irvine , CA , USA.
出版信息
Islets. 2018 Jan 2;10(1):40-49. doi: 10.1080/19382014.2017.1405202. Epub 2018 Jan 9.
Pancreatic islet transplantation is being extensively researched as an alternative treatment for type 1 diabetic patients. This treatment is currently limited by temporal mismatch, between the availability of pancreas and isolated islets from deceased organ donor, and the recipient's need for freshly isolated islets. To solve this issue, cryopreservation of islets may offer the potential to bank islets for transplant on demand. Cryopreservation, however, introduces an overwhelmingly harsh environment to the ever-so-fragile islets. After exposure to the freezing and thawing, islets are usually either apoptotic, non-functional, or non-viable. Several studies have proposed various techniques that could lead to increased cell survival and function following a deep freeze. The purpose of this article is to critically review the techniques of islet cryopreservation, with the goal of highlighting optimization parameters that can lead to the most viable and functional islet upon recovery and/or transplant.
胰岛移植作为1型糖尿病患者的替代治疗方法正在被广泛研究。目前，这种治疗受到时间不匹配的限制，即来自已故器官捐赠者的胰腺和分离的胰岛的可用性与接受者对新鲜分离胰岛的需求之间的不匹配。为了解决这个问题，胰岛的冷冻保存可能为按需储存胰岛用于移植提供潜力。然而，冷冻保存给极其脆弱的胰岛带来了极其恶劣的环境。在经历冷冻和解冻后，胰岛通常要么凋亡、无功能，要么无法存活。几项研究提出了各种技术，这些技术可以在深度冷冻后提高细胞存活率和功能。本文的目的是批判性地回顾胰岛冷冻保存技术，目标是突出那些在恢复和/或移植时能够产生最具活力和功能的胰岛的优化参数。
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