Department of Virology, National Influenza Center, Respiratory Viruses Unit, Institut Pasteur of Algeria, Algiers, Algeria.
Faculty of Sciences, Department of Biology, EcoSTAq Research Laboratory, University Badji Mokhtar, Annaba, Algeria.
J Med Virol. 2018 May;90(5):844-853. doi: 10.1002/jmv.25029. Epub 2018 Jan 30.
Influenza season 2007/2008 was marked by a worldwide emergence of oseltamivir-resistant A(H1N1) viruses possessing a mutation in the neuraminidase gene causing His-to-Tyr substitution at amino acid position 275 (H275Y). These strains were isolated in Algeria where 30% of seasonal A(H1N1) viruses harbored the H275Y mutation. Emergence of resistant viruses to currently approved antiviral drug determined the need for antiviral susceptibility monitoring in Algeria especially that oseltamivir is currently used in hospitals of some provinces of the country for treatment of influenza in populations at risk. The aim of the present study is to investigate the sensitivity of circulating influenza viruses in Algeria to oseltamivir. We present 5-year local surveillance results from 2009/2010 influenza season to 2013/2014 influenza season. We tested the sensitivity to oseltamivir of 387 human influenza A and B viruses isolated in Algeria. Determination of IC values were performed using the fluorogenic MUNANA substrate. To detect the H275Y mutation in the neuraminidase of the A(H1N1) strains we performed a real-time RT-PCR allelic discrimination analysis. The obtained results showed that all influenza A(H1N1)pdm09, A(H3N2), and B viruses studied remained susceptible to oseltamivir. This is the first study on influenza antiviral susceptibility surveillance in Algeria. Obtained results allow establishing a baseline data for future studies on antiviral resistance emergence worldwide. Our report highlights the importance of a continued and active monitoring of circulating viruses in Algeria for strengthens collaboration within the Global Influenza Surveillance and Response System.
2007/2008 年流感季节在全球范围内出现了神经氨酸酶基因发生突变的奥司他韦耐药 A(H1N1)病毒，导致氨基酸位置 275 处的组氨酸到酪氨酸取代（H275Y）。这些菌株是在阿尔及利亚分离出来的，那里 30%的季节性 A(H1N1)病毒携带 H275Y 突变。目前批准的抗病毒药物的耐药病毒的出现决定了在阿尔及利亚进行抗病毒敏感性监测的必要性，特别是奥司他韦目前在该国一些省份的医院中用于治疗高危人群的流感。本研究的目的是调查在阿尔及利亚循环流感病毒对奥司他韦的敏感性。我们展示了 2009/2010 流感季节至 2013/2014 流感季节的 5 年本地监测结果。我们测试了在阿尔及利亚分离的 387 株人甲型和乙型流感病毒对奥司他韦的敏感性。使用荧光 MUNANA 底物测定 IC 值。为了检测 A(H1N1)pdm09、A(H3N2)和 B 型流感病毒的神经氨酸酶中的 H275Y 突变，我们进行了实时 RT-PCR 等位基因区分分析。结果表明，所有研究的 A(H1N1)pdm09、A(H3N2)和 B 型流感病毒均对奥司他韦敏感。这是阿尔及利亚首次进行流感抗病毒敏感性监测研究。获得的结果允许为未来在全球范围内开展抗药性出现的研究建立基线数据。我们的报告强调了在阿尔及利亚继续积极监测循环病毒的重要性，以加强全球流感监测和应对系统内的合作。
