School of Food and Nutritional Sciences, University College Cork, Ireland.
School of Food and Nutritional Sciences, University College Cork, Ireland; APC Microbiome Institute, Ireland.
Int J Food Microbiol. 2018 Mar 2;268:19-26. doi: 10.1016/j.ijfoodmicro.2018.01.001. Epub 2018 Jan 4.
The aim of this study was to develop a novel beverage fermented with Weissella cibaria MG1 based on aqueous extracts of wholemeal quinoa flour. The protein digestibility of quinoa based-milk was improved by applying complex proteolytic enzymes able to increase protein solubility by 54.58%. The growth and fermentation characteristics of Weissella cibaria MG1, including EPS production at the end of fermentation, were investigated. Fermented wholemeal quinoa milk using MG1 showed high viable cell counts (>10cfu/ml), a pH of 5.16, and significantly higher water holding capacity (WHC, 100%), viscosity (0.57mPas) and exopolysaccharide (EPS) amount (40mg/l) than the chemical acidified control. High EPS (dextran) concentration in quinoa milk caused earlier aggregation because more EPS occupy more space, and the chenopodin were forced to interact with each other. Microstructure observation indicated that the network structures of EPS-protein improve the texture of fermented quinoa milk. Overall, Weissella cibaria MG1 showed satisfactory technology properties and great potential for further possible application in the development of high viscosity fermented quinoa milk.
本研究旨在以全麦藜麦粉的水提物为基础,开发一种基于魏斯氏菌 MG1 的新型发酵饮料。通过应用能够提高蛋白质溶解度 54.58%的复合蛋白酶,提高了藜麦乳的蛋白质消化率。研究了魏斯氏菌 MG1 的生长和发酵特性,包括发酵结束时 EPS 的生产。使用 MG1 发酵全麦藜麦奶显示出较高的活菌数(>10cfu/ml)、pH 值为 5.16,并且持水能力(WHC,100%)、粘度(0.57mPas)和胞外多糖(EPS)含量(40mg/l)显著高于化学酸化对照。藜麦乳中高浓度的 EPS(葡聚糖)导致更早的聚集,因为更多的 EPS 占据更多的空间,而 chenopodin 被迫相互作用。微观结构观察表明,EPS-蛋白质的网络结构改善了发酵藜麦乳的质地。总的来说,魏斯氏菌 MG1 表现出令人满意的技术特性,具有进一步开发高粘度发酵藜麦乳的巨大潜力。