Søndergaard F, Kristensen M, Tommerup N
Prenat Diagn. 1985 Jul-Aug;5(4):291-4. doi: 10.1002/pd.1970050408.
A simple method for obtaining prometaphase chromosomes from cultured first trimester cells involves the addition of BrdU and FdU 11 h before harvest and ethidium bromide 1.5 h before harvest. High resolution R-banding is obtained by acridine orange staining.
一种从孕早期培养细胞中获取前中期染色体的简单方法,包括在收获前11小时添加溴脱氧尿苷(BrdU)和氟脱氧尿苷(FdU),以及在收获前1.5小时添加溴化乙锭。通过吖啶橙染色获得高分辨率R带。