Department of Pathobiology, Ontario Veterinary College, University of Guelph, 50 Stone Road East, Guelph N1G 2W1, Ontario, Canada.
Department of Pathobiology, Ontario Veterinary College, University of Guelph, 50 Stone Road East, Guelph N1G 2W1, Ontario, Canada.
Genomics. 2019 Jan;111(1):59-66. doi: 10.1016/j.ygeno.2018.01.001. Epub 2018 Jan 6.
Actinobacillus spp. are Gram-negative bacteria associated with mucosal membranes. While some are commensals, others can cause important human and animal diseases. A. pleuropneumoniae causes severe fibrinous hemorrhagic pneumonia in swine but not systemic disease whereas other species invade resulting in septicemia and death. To understand the invasive phenotype of Actinobacillus spp., complete genomes of eight isolates were obtained and pseudogenomes of five isolates were assembled and annotated. Phylogenetically, A. suis isolates clustered by surface antigen type and were more closely related to the invasive A. ureae, A. equuli equuli, and A. capsulatus than to the other swine pathogen, A. pleuropneumoniae. Using the LS-BSR pipeline, 251 putative virulence genes associated with serum resistance and invasion were detected. To our knowledge, this is the first genome-wide study of the genus Actinobacillus and should contribute to a better understanding of host tropism and mechanisms of invasion of pathogenic Actinobacillus and related genera.
放线杆菌属是与黏膜相关的革兰氏阴性细菌。有些是共生菌，而有些则会导致重要的人类和动物疾病。胸膜肺炎放线杆菌会导致猪严重的纤维蛋白性出血性肺炎，但不会引起全身性疾病，而其他物种则会入侵导致败血症和死亡。为了了解放线杆菌属的侵袭表型，我们获得了 8 个分离株的全基因组，并组装和注释了 5 个分离株的伪基因组。从系统发育上看，猪源分离株根据表面抗原类型聚类，与侵袭性的解脲放线杆菌、马驹放线杆菌和囊包放线杆菌的亲缘关系比其他猪病原体胸膜肺炎放线杆菌更近。使用 LS-BSR 管道，检测到 251 个与血清抗性和侵袭相关的假定毒力基因。据我们所知，这是放线杆菌属的全基因组研究，应该有助于更好地了解致病性放线杆菌属和相关属的宿主嗜性和侵袭机制。
