Rakhshanpour Alaleh, Malmasi Abdolali, Mohebali Mehdi, Nabian Sedigheh, Mirhendi Hossein, Zarei Zabihollah, Dalimi Abdolhossein, Mohammadiha Anita, Akhoundi Behnaz, Azarm Amrollah
Dept. of Internal Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
Dept. of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Iran J Parasitol. 2017 Oct-Dec;12(4):482-489.
is the most widely distributed tick in the world, which is partly due to its biological flexibility and the global distribution of its major host, the domestic dog. In Mediterranean region it could be principal reservoir host for usually transmitted by the phlebotomine sand flies. In this study, we evaluated the vector potential of in transmitting to uninfected dogs.
During 2014, five dogs with clinical manifestations of canine visceral leishmaniasis (CVL), high anti- antibody titers and tick infestation, were selected from CVL endemic areas (Tehran and Alborz provinces). At least, twenty live ticks were removed from each infected dog. After morphological identification, the ticks were divided into two groups; ticks belonging to the first group were dissected for parasitological examinations and semi-nested PCR assay, and those of the second group were selected for the transmission of CVL caused by to uninfected dogs. Following tick infestation, all uninfected dogs were kept for 9 months and examined monthly for clinical and serological tests.
Nearly, 67% of ticks were infected by using the semi-nested PCR. All other parasitological tests of ticks were negative. Clinical examinations and serological tests of the investigated dogs revealed negative results. Nested-PCR test results performed on splenic biopsy samples of dogs were also negative.
-positive ticks were unable to transfer from infected dogs to healthy ones. The detection of DNA in ticks collected from naturally infected dogs by semi-nested PCR does not prove their vectorial competence.
是世界上分布最广的蜱虫，部分原因在于其生物学适应性以及其主要宿主家犬在全球的分布。在地中海地区，它可能是通常由白蛉传播的病原体的主要储存宿主。在本研究中，我们评估了蜱虫将病原体传播给未感染犬只的媒介潜力。
2014年期间，从犬内脏利什曼病（CVL）流行地区（德黑兰和阿尔伯兹省）挑选了五只表现出CVL临床表现、抗抗体滴度高且有蜱虫寄生的犬只。从每只感染犬只身上至少采集20只活蜱。经过形态学鉴定后，将蜱虫分为两组；第一组的蜱虫进行解剖以进行寄生虫学检查和半巢式PCR检测，第二组的蜱虫用于将由病原体引起的CVL传播给未感染的犬只。蜱虫寄生后，所有未感染的犬只饲养9个月，并每月进行临床和血清学检测。
使用半巢式PCR检测发现，近67%的蜱虫感染了病原体。蜱虫的所有其他寄生虫学检测均为阴性。对所调查犬只的临床检查和血清学检测结果均为阴性。对犬只脾脏活检样本进行的巢式PCR检测结果也为阴性。
携带病原体阳性的蜱虫无法将病原体从感染犬只传播给健康犬只。通过半巢式PCR从自然感染犬只采集的蜱虫中检测到病原体DNA并不能证明它们具有媒介能力。
