School of Pharmacy, Aichi Gakuin University, 1-100 Kusumoto-cho, Chikusa-ku, Nagoya, 464-8650, Japan.
School of Dentistry, Aichi Gakuin University, 1-100 Kusumoto-cho, Chikusa-ku, Nagoya, 464-8650, Japan.
Mol Cell Biochem. 2018 Sep;446(1-2):83-89. doi: 10.1007/s11010-018-3275-2. Epub 2018 Jan 9.
Autonomic neurons innervate pancreatic islets of Langerhans and participate in the maintenance of blood glucose concentrations by controlling hormone levels through attachment with islet cells. We previously found that stimulated superior cervical ganglia (SCG) could induce Ca oscillation in α cells via neuropeptide substance P using an in vitro co-culture model. In this study, we studied the effect of SCG neurite adhesion on intracellular secretory granule movement and glucagon secretion in α cells stimulated by low glucose concentration. Spinning disk microscopic analysis revealed that the mean velocity of intracellular granules was significantly lower in α cells attached to SCG neurites than that in those without neurites under low (2 mM), middle (10 mM), and high (20 mM) glucose concentrations. Stimulation by a low (2 mM) glucose concentration significantly increased glucagon secretion in α cells lacking neurites but not in those bound to neurites. These results suggest that adhesion to SCG neurites decreases low glucose-induced glucagon secretion in pancreatic α cells by attenuating intracellular granule movement activity.
自主神经元支配胰岛的朗格汉斯岛，并通过与胰岛细胞的附着来控制激素水平，从而参与维持血糖浓度。我们之前发现，通过体外共培养模型，刺激颈上神经节 (SCG) 可以通过神经肽 P 物质诱导α细胞中的 Ca 振荡。在这项研究中，我们研究了 SCG 神经突黏附对低葡萄糖浓度刺激下α细胞内分泌颗粒运动和胰高血糖素分泌的影响。旋转盘显微镜分析显示，在低（2mM）、中（10mM）和高（20mM）葡萄糖浓度下，与 SCG 神经突附着的α细胞内颗粒的平均速度明显低于没有神经突的α细胞。低（2mM）葡萄糖浓度刺激显著增加了缺乏神经突的α细胞中胰高血糖素的分泌，但对与神经突结合的α细胞没有影响。这些结果表明，与 SCG 神经突的黏附通过减弱细胞内颗粒运动活性，降低了低葡萄糖诱导的胰岛α细胞中胰高血糖素的分泌。
