人基质金属蛋白酶催化结构域在大肠杆菌周质中的功能性表达
Functional Production of Catalytic Domains of Human MMPs in Escherichia coli Periplasm.
作者信息
Nam Dong Hyun, Lee Ki Baek, Ge Xin
机构信息
Department of Chemical and Environmental Engineering, University of California, Riverside, CA, USA.
出版信息
Methods Mol Biol. 2018;1731:65-72. doi: 10.1007/978-1-4939-7595-2_7.
Abstract
Due to their central roles in tumor growth and invasion, milligram-level amounts of active MMPs are frequently required for cancer research and development of chemical or biological MMP inhibitors. Here we describe methods for functional production of catalytic domains of MMPs (cdMMPs) in E. coli periplasm without refolding or activation process. We demonstrate applications of this straightforward approach for cdMMP-9, cdMMP-14, and cdMMP-14 mutants.
摘要
由于基质金属蛋白酶（MMPs）在肿瘤生长和侵袭中发挥核心作用，癌症研究以及化学或生物MMP抑制剂的开发常常需要毫克级量的活性MMPs。在此，我们描述了在大肠杆菌周质中功能性生产MMPs催化结构域（cdMMPs）的方法，无需复性或激活过程。我们展示了这种直接方法在cdMMP-9、cdMMP-14cdMMP-14和cdMMP-14突变体中的应用。
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