Åström Pirjo, Heljasvaara Ritva, Nyberg Pia, Al-Samadi Ahmed, Salo Tuula
Cancer and Translational Medicine Research Unit, University of Oulu, Oulu, Finland.
Medical Research Center, Oulu University Hospital, Oulu, Finland.
Methods Mol Biol. 2018;1731:213-221. doi: 10.1007/978-1-4939-7595-2_19.
Here we describe a protocol to utilize human benign leiomyoma tissue in in vitro 3D model that enables an assessment of cell invasion. The chapter also describes detailed instructions for image analysis to quantify the results. Leiomyoma is a benign tumor of the uterus which mimics authentic components of the tumor microenvironment including fibroblasts, vessels, collagen fibers, and extracellular protein composition. The leiomyoma invasion model represents a superior 3D model for cell invasion studies compared to the other non-human organotypic models.
在此，我们描述了一种在体外3D模型中利用人良性平滑肌瘤组织的方案，该模型能够评估细胞侵袭。本章还介绍了用于量化结果的图像分析的详细说明。平滑肌瘤是子宫的一种良性肿瘤，它模拟肿瘤微环境的真实成分，包括成纤维细胞、血管、胶原纤维和细胞外蛋白质组成。与其他非人体器官型模型相比，平滑肌瘤侵袭模型是一种用于细胞侵袭研究的优越3D模型。
