高选择性基质金属蛋白酶抗体抑制剂的生成。
Generation of Highly Selective MMP Antibody Inhibitors.
作者信息
Nam Dong Hyun, Ge Xin
机构信息
Department of Chemical and Environmental Engineering, University of California, Riverside, CA, USA.
出版信息
Methods Mol Biol. 2018;1731:307-324. doi: 10.1007/978-1-4939-7595-2_26.
Abstract
Inhibiting individual MMPs of biomedical importance with high selectivity is critical for both fundamental research and therapy development. Here we describe the methods for discovery of inhibitory monoclonal antibodies from synthetic human antibody phage display libraries carrying convex paratopes encoded by long complementarity-determining region (CDR)-H3 segments. We demonstrate the application of this technique for isolation of highly specific and potent antibody inhibitors of human MMP-14.
摘要
以高选择性抑制具有生物医学重要性的单个基质金属蛋白酶（MMP）对于基础研究和治疗开发都至关重要。在此，我们描述了从携带由长互补决定区（CDR）-H3片段编码的凸型互补决定区的合成人抗体噬菌体展示文库中发现抑制性单克隆抗体的方法。我们展示了该技术在分离人MMP-14的高度特异性和强效抗体抑制剂中的应用。
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