胚胎发育至囊胚阶段，第3天有无更换单一培养基。
Embryo development until blastocyst stage with and without renewal of single medium on day 3.
作者信息
Peña Fernando, Dávalos Rocío, Rechkemmer Adolfo, Ascenzo Alvaro, Gonzales Mauricio
机构信息
Laboratorio de Reproducción Asistida, Clínica Miraflores, Lima, Peru.
Laboratorio de Biotecnología Animal, Facultad de Ciencias, Universidad Ricardo Palma, Lima, Peru.
出版信息
JBRA Assist Reprod. 2018 Mar 1;22(1):49-51. doi: 10.5935/1518-0557.20180012.
OBJECTIVE
This study aimed to assess the effects of using a single culture medium not renewed on day 3 on the development of the embryo up to the blastocyst stage.
METHODS
The study was carried out in the Assisted Reproduction Laboratory of Clínica Miraflores in the period ranging from April to December of 2016. The study included 589 human embryos obtained from 82 couples submitted to IVF/ICSI with donor oocytes. The couples were randomly divided into two groups: group 1 (medium renewed on day 3) and group 2 (medium not renewed on day 3).
RESULTS
Significant differences in pregnancy rates, implantation rates, and blastocyst formation were not observed.
CONCLUSION
No statistically significant difference was found between the groups with respect to the analyzed parameters. However, a non-significant trend was observed in favor of the group without medium renewal for clinical pregnancy and positive pregnancy rates (β-hCG).
目的
本研究旨在评估使用第3天不更换的单一培养基对胚胎发育至囊胚阶段的影响。
方法
该研究于2016年4月至12月在米拉弗洛雷斯诊所的辅助生殖实验室进行。研究纳入了从82对接受体外受精/卵胞浆内单精子注射（IVF/ICSI）并使用供体卵母细胞的夫妇中获得的589个人类胚胎。这些夫妇被随机分为两组：第1组（第3天更换培养基）和第2组（第3天不更换培养基）。
结果
未观察到妊娠率、着床率和囊胚形成方面的显著差异。
结论
在分析的参数方面，两组之间未发现统计学上的显著差异。然而，观察到一种不显著的趋势，即不更换培养基的组在临床妊娠和阳性妊娠率（β-hCG）方面更具优势。
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