Amirfakhrian Hossein, Abedi Seyed Mohammad, Sadeghi Hossein, Azizi Soheil, Hosseinimehr Seyed Jalal
Department of Radiopharmacy, Mazandaran University of Medical Sciences, Sari, Iran.
Nucl Med Rev Cent East Eur. 2018;21(1):8-13. doi: 10.5603/NMR.a2018.0006. Epub 2018 Jan 10.
In this study, we investigated the protective effect of vitamin E against methotrexate (MTX)-induced hepatotoxicity by quantitative liver 99mTc-phytate uptake and liver imaging and to compare its effect with histopathology in rat. Rats were divided into five groups as control, solvent, Vit E (100 mg/kg), MTX (20 mg/kg), Vit E + MTX and. Vit E was intraperitoneally administrated for 17 days before MTX injection and continued for 4 days. 99mTc-phytate was injected through the tail of rats after the drug administration. The percentage of the injected dose per gram of liver and spleen tissues (%ID/g) was calculated. Liver imaging was obtained with gamma camera. In other experiment, liver of treated rats were assessed for histopathology. 99mTc-phytate uptake per gram tissue of the livers as %ID/g in control, solvent, MTX, Vit E, Vit E + MTX and MTX groups were 8.99% ± 1.37, 8.53% ± 2.91, 8.65% ± 3.84, 3.22% ± 1.09 and 8.38% ± 2.68. Vit E administration with MTX resulted in a significant increasing in the level of %ID/g. Vit E treatment improved the shape of live in planner image. Histophatological examinations showed a protective effect of Vit E against MTX-induced hepatoxicity in rats. The results showed that Vit E significantly attenuates the MTX-induced hepatotoxicity in rats, and 99mTc-phytate uptake in liver as well as liver image to be acceptable techniques for assessment of liver and spleen damages and/or their tissues protective effects in animal model.
在本研究中，我们通过定量肝脏99mTc-植酸盐摄取和肝脏成像来研究维生素E对甲氨蝶呤（MTX）诱导的肝毒性的保护作用，并将其与大鼠组织病理学结果进行比较。大鼠被分为五组，即对照组、溶剂组、维生素E（100mg/kg）组、MTX（20mg/kg）组、维生素E+MTX组。在注射MTX前17天腹腔注射维生素E，并持续4天。给药后经大鼠尾部注射99mTc-植酸盐。计算每克肝脏和脾脏组织的注射剂量百分比（%ID/g）。用γ相机进行肝脏成像。在另一项实验中，对处理过的大鼠肝脏进行组织病理学评估。对照组、溶剂组、MTX组、维生素E组、维生素E+MTX组和MTX组肝脏每克组织的99mTc-植酸盐摄取量（%ID/g）分别为8.99%±1.37、8.53%±2.91、8.65%±3.84、3.22%±1.09和8.38%±2.68。维生素E与MTX联合给药导致%ID/g水平显著升高。维生素E治疗改善了平面图像中肝脏的形态。组织病理学检查显示维生素E对大鼠MTX诱导的肝毒性具有保护作用。结果表明，维生素E能显著减轻大鼠MTX诱导的肝毒性，肝脏99mTc-植酸盐摄取以及肝脏成像可作为评估动物模型中肝脏和脾脏损伤及/或其组织保护作用的可接受技术。
