Departamento de Bioquímica y Biología Molecular, Universidad de Sevilla, Sevilla, Spain.
Departamento de Fisiología, Universidad de Sevilla, Sevilla, Spain.
J Cell Physiol. 2018 Oct;233(10):6317-6328. doi: 10.1002/jcp.26439. Epub 2018 Apr 25.
Adipose tissue is an important source of adipose derived stem cells (ADSCs). These cells have the potential of being used for certain therapies, in which the main objective is to recover the function of a tissue/organ affected by a disease. In order to contribute to repair of the tissue, these cells should be able to survive and carry out their functions in unfavorable conditions after being transplanted. This process requires a better understanding of the biology involved: such as the time cells remain in the implant site, how long they stay there, and whether or not they differentiate into host tissue cells. This report focuses on these questions. ADSC were injected into three different tissues (substantia nigra, ventricle, liver) and they were tracked in vivo with a dual GFP-Luc reporter system. The results show that ADSCs were able to survive up to 4 months after the engraftment and some of them started showing resident cell tissue phenotype. These results demonstrate their long-term capacity of survival and differentiation when injected in vivo.
脂肪组织是脂肪来源干细胞（ADSCs）的重要来源。这些细胞有可能被用于某些治疗方法，其主要目的是恢复受疾病影响的组织/器官的功能。为了有助于组织修复，这些细胞应该能够在被移植后在不利条件下存活并发挥其功能。这个过程需要更好地了解所涉及的生物学：例如细胞在植入部位停留的时间，它们在那里停留的时间长短，以及它们是否分化为宿主组织细胞。本报告重点介绍了这些问题。将 ADSC 注入到三个不同的组织（黑质、脑室、肝脏）中，并使用双 GFP-Luc 报告系统在体内进行跟踪。结果表明，ADSC 在移植后能够存活长达 4 个月，并且其中一些开始表现出常驻细胞组织表型。这些结果表明它们在体内注射时具有长期的存活和分化能力。
