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分离的肌浆网细胞质表面上Ca2+ -ATP酶的有序阵列。

Ordered arrays of Ca2+-ATPase on the cytoplasmic surface of isolated sarcoplasmic reticulum.

作者信息

Ferguson D G, Franzini-Armstrong C, Castellani L, Hardwicke P M, Kenney L J

出版信息

Biophys J. 1985 Oct;48(4):597-605. doi: 10.1016/S0006-3495(85)83815-7.

DOI:10.1016/S0006-3495(85)83815-7
PMID:2932170
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1329336/
Abstract

Isolated sarcoplasmic reticulum (SR) vesicles with polymerized calcium pump protein were freeze-dried and rotary shadowed following uranyl acetate stabilization. This technique allows direct observation of a single side of the vesicle without requiring optical filtering. The heads of individual ATPase molecules, projecting above the cytoplasmic surface, are clearly resolved in the replicas. Ca ATPase molecules form extensive arrays in vanadate-treated, rabbit SR vesicles and in gently isolated, native SR vesicles from scallop. Gentle isolation results in limited areas of orderly structure in native SR isolated from vertebrate muscles. Special attention is given to the effect of various shadow thicknesses on the appearance of the heads. This information is essential to the interpretation of images in the accompanying paper (Franzini-Armstrong, C., and D.J. Ferguson, 1985, Biophys. J., 48:607-615).

摘要

将含有聚合钙泵蛋白的分离肌质网（SR）囊泡用醋酸铀稳定后进行冷冻干燥和旋转投影。该技术可直接观察囊泡的单面，无需光学滤波。在复制品中可以清楚地分辨出突出于细胞质表面的单个ATP酶分子的头部。Ca ATP酶分子在经钒酸盐处理的兔SR囊泡以及从扇贝中轻度分离的天然SR囊泡中形成广泛的阵列。轻度分离导致从脊椎动物肌肉中分离出的天然SR中有序结构的区域有限。特别关注了各种投影厚度对头部外观的影响。这些信息对于解释随附论文中的图像至关重要（Franzini-Armstrong, C., and D.J. Ferguson, 1985, Biophys. J., 48:607-615）。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53af/1329336/a92c740e49b9/biophysj00187-0061-d.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53af/1329336/36aebe7fa8a4/biophysj00187-0057-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53af/1329336/e4ce8765b751/biophysj00187-0057-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53af/1329336/ac2a0cb4ebc1/biophysj00187-0057-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53af/1329336/08ac37934a2d/biophysj00187-0058-a.jpg
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Fluorescence energy transfer as an indicator of Ca2+-ATPase interactions in sarcoplasmic reticulum.荧光能量转移作为肌浆网中Ca2 + -ATP酶相互作用的指标。
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Effect of phosphorylation on scallop sarcoplasmic reticulum.磷酸化对扇贝肌浆网的影响。
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