肌浆网的液体衍射分析。II. 溶剂电子对比度变化
Liquid diffraction analysis of sarcoplasmic reticulum. II. Solvent electron contrast variation.
作者信息
Brady G W, Fein D B, Meissner G, Harder M E
出版信息
Biophys J. 1982 Mar;37(3):637-45.
Abstract
Intensities of x-ray scattering from rabbit muscle sarcoplasmic reticulum membrane have been measured over the range of s = 0.05-0.25, at solvent densities varying between p0 = 0.335 and 0.389 electrons/A3. Analysis of the results shows agreement with the elements of structure deduced earlier by a different technique, based on the variation of the lipid-protein concentration ratio. In addition, the present work extends the analysis to allow isolation of the lipid contribution to the total scattering, from which a profile of the lipid electron density normal to the membrane face is evaluated. The scattering arising from electron correlations within the plane of the bilayer has also been identified.
摘要
在溶剂密度介于(p0 = 0.335)和(0.389)电子/ų之间时，测量了兔肌浆网膜在(s = 0.05 - 0.25)范围内的X射线散射强度。结果分析表明，与早期基于脂质 - 蛋白质浓度比变化采用不同技术推导的结构要素一致。此外，本研究扩展了分析，以便分离脂质对总散射的贡献，据此评估垂直于膜面的脂质电子密度分布。还识别出了双层平面内电子相关性产生的散射。
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