College of Food Science and Engineering, Yangzhou University, Yangzhou 225127, Jiangsu, China.
Food Funct. 2018 Feb 21;9(2):937-950. doi: 10.1039/c7fo01302g.
In this study, three polysaccharides including water-soluble polysaccharide (WSP), dilute alkali-soluble polysaccharide (DASP) and concentrated alkali-soluble polysaccharide (CASP) were extracted from purple sweet potatoes and then administered to normal and cyclophosphamide (CTX) treated mice by gavage. The results showed that WSP and CASP could restore the spleen index and immune cytokine (IL-2 and IL-6) levels in CTX treated mice, while DASP could enhance the levels of TNF-α, IL-2 and IL-6. As compared to the normal control group, WSP and CASP treatment groups exhibited increased levels of Bacteroidetes, Lachnospiraceae and Oscillospira, but decreased levels of Firmicutes, Alcaligenaceae and Sutterella in normal mice. When compared with a model control group, all the three polysaccharide treatment groups showed relatively higher abundances of Bacteroidetes, Ruminococcaceae, Lachnospiraceae, Ruminococcus and Oscillospira but lower levels of Firmicutes, Proteobacteria, Alcaligenaceae and Sutterella in CTX treated mice. Moreover, all the polysaccharides could enhance the production of acetic acid, propionic acid and butyric acid in normal mice, while WSP could upregulate the production of these short chain fatty acids in CTX treated mice.
在这项研究中，从紫薯中提取了三种多糖，包括水溶性多糖（WSP）、稀碱溶性多糖（DASP）和浓碱溶性多糖（CASP），然后通过灌胃给药给正常和环磷酰胺（CTX）处理的小鼠。结果表明，WSP 和 CASP 可恢复 CTX 处理小鼠的脾指数和免疫细胞因子（IL-2 和 IL-6）水平，而 DASP 可增强 TNF-α、IL-2 和 IL-6 的水平。与正常对照组相比，WSP 和 CASP 处理组的拟杆菌门、lachnospiraceae 和 Oscillospira 水平升高，但正常小鼠的厚壁菌门、Alcaligenaceae 和 Sutterella 水平降低。与模型对照组相比，三种多糖处理组在 CTX 处理小鼠中均表现出相对较高的拟杆菌门、Ruminococcaceae、lachnospiraceae、Ruminococcus 和 Oscillospira 的丰度，而Firmicutes、Proteobacteria、Alcaligenaceae 和 Sutterella 的丰度较低。此外，所有多糖均可增强正常小鼠中乙酸、丙酸和丁酸的产生，而 WSP 可上调 CTX 处理小鼠中这些短链脂肪酸的产生。
