Pánková Hana, Lepinay Clémentine, Rydlová Jana, Voříšková Alena, Janoušková Martina, Dostálek Tomáš, Münzbergová Zuzana
Institute of Botany, The Czech Academy of Sciences, Zámek 1, 252 43, Průhonice, Czech Republic.
Department of Experimental Plant Biology, Faculty of Science, Charles University, Viničná 5, 128 00, Prague, Czech Republic.
Oecologia. 2018 Mar;186(3):677-689. doi: 10.1007/s00442-017-4054-6. Epub 2018 Jan 10.
After abandonment of agricultural fields, some grassland plant species colonize these sites with a frequency equivalent to dry grasslands (generalists) while others are missing or underrepresented in abandoned fields (specialists). We aimed to understand the inability of specialists to spread on abandoned fields by exploring whether performance of generalists and specialists depended on soil abiotic and/or biotic legacy. We performed a greenhouse experiment with 12 species, six specialists and six generalists. The plants were grown in sterile soil from dry grassland or abandoned field inoculated with microbial communities from one or the other site. Plant growth, abundance of mycorrhizal structures and plant response to inoculation were evaluated. We focused on arbuscular mycorrhizal fungi (AMF), one of the most important parts of soil communities affecting plant performance. The abandoned field soil negatively affected plant growth, but positively affected plant response to inoculation. The AMF community from both sites differed in infectivity and taxa frequencies. The lower AMF taxa frequency in the dry grassland soil suggested a lack of functional complementarity. Despite the fact that dry grassland AMF produced more arbuscules, the dry grassland inoculum did not improve phosphorus nutrition of specialists contrary to the abandoned field inoculum. Inoculum origin did not affect phosphorus nutrition of generalists. The lower effectiveness of the dry grassland microbial community toward plant performance excludes its inoculation in the abandoned field soil as a solution to allow settlement of specialists. Still, the distinct response of specialists and generalists to inoculation suggested that they differ in AMF responsiveness.
在农田被弃耕后，一些草原植物物种以与干旱草原相当的频率在这些地点定殖（通才物种），而其他物种在弃耕地上缺失或数量不足（专才物种）。我们旨在通过探究通才物种和专才物种的表现是否取决于土壤非生物和/或生物遗留物，来理解专才物种无法在弃耕地上扩散的原因。我们用12个物种进行了一项温室实验，其中6个是专才物种，6个是通才物种。这些植物种植在来自干旱草原或弃耕地的无菌土壤中，并接种来自其中一个地点的微生物群落。评估了植物生长、菌根结构的丰度以及植物对接种的反应。我们关注丛枝菌根真菌（AMF），它是影响植物表现的土壤群落的最重要部分之一。弃耕地土壤对植物生长有负面影响，但对接种反应有积极影响。来自两个地点的AMF群落的感染力和分类群频率不同。干旱草原土壤中较低的AMF分类群频率表明缺乏功能互补性。尽管干旱草原的AMF产生了更多的丛枝，但与弃耕地接种物相反，干旱草原接种物并没有改善专才物种的磷营养。接种物来源不影响通才物种的磷营养。干旱草原微生物群落对植物表现的较低有效性排除了将其接种到弃耕地土壤中作为让专才物种定居的解决方案。然而，专才物种和通才物种对接种的不同反应表明它们在AMF反应性方面存在差异。
