Nakamura T, Kuroda T, Noguchi K, Funaki J
Bull Tokyo Med Dent Univ. 1979 Dec;26(4):273-8.
The purpose of this experiment was to examine the growth of condylar, epiphyseal, and spheno-occipital synchrondrosal cartilages cultured on medium BGJ. Materials from 12 neonatal rabbits were cultured for 7 days on this medium with the addition of 0, 10, or 20% fetal calf serum. Epiphyseal cartilages cultured with the addition of 20% fetal calf serum showed slightly better maintenance of in situ state, though little morphological changes were observed during 7 days. Spheno-occipital synchondrosal cartilages cultured with the addition of 10% or 20% fetal calf serum were histologically relatively similar to in situ state than those cultured without fetal calf serum, but no growth in length and width of these cartilages was observed. On the other hand, cultured condylar cartilages showed the least maintenance of histological and histochemical features. It was assumed that medium BGJ was not a recommendable medium for the organ culture of condylar cartilage.
本实验的目的是研究在BGJ培养基上培养的髁突软骨、骨骺软骨和蝶枕软骨联合的生长情况。取自12只新生兔的材料在添加了0%、10%或20%胎牛血清的该培养基上培养7天。添加20%胎牛血清培养的骨骺软骨在原位状态的维持上稍好一些,不过在7天内未观察到明显的形态学变化。添加10%或20%胎牛血清培养的蝶枕软骨联合在组织学上比未添加胎牛血清培养的更接近原位状态,但未观察到这些软骨的长度和宽度增长。另一方面,培养的髁突软骨在组织学和组织化学特征的维持上表现最差。据推测,BGJ培养基并非髁突软骨器官培养的推荐培养基。