Iguchi Y, Tokuda H, Tamura S, Kishioka S, Ozaki M, Yamamoto H
Nihon Yakurigaku Zasshi. 1985 Aug;86(2):105-14. doi: 10.1254/fpj.86.105.
Effects of electroacupuncture (EA) on pain threshold and beta-endorphin (beta-End) contents in plasma, pituitary (Pit), hypothalamus (Hyp) and cerebrospinal fluid (CSF) were studied in nontreated, dexamethasone (Dex) treated and adrenalectomized (Adrex) male SD rats by the use of specific determination of rat beta-End (combination of HPLC and RIA). EA increased pain threshold and plasma beta-End with no effect on beta-End contents in Pit, Hyp and CSF. Dex did not affect control pain threshold, but tended to reduce EA-induced increase in pain threshold (EA-analgesia, EAA) and EA-induced increase in plasma beta-End. Adrex increased plasma beta-End without change in control pain threshold. Adrex tended to reduce EAA, but a tendency of further increase in plasma beta-End was observed after addition of EA. Adrex increased Pit beta-End, but no further change in Pit beta-End was observed after addition of EA. A positive correlation between plasma beta-End and plasma ACTH was observed in nontreated, Dex treated and Adrex rats. No correlation between plasma beta-End and potency of EAA was observed in nontreated, Dex treated and Adrex rats. The hind-paw pressure test without EA increased plasma beta-End to the same degree as that produced by EA, and it produced no analgesia. These results suggest that Pit beta-End may not be mainly involved in the development of EAA.
通过使用大鼠β-内啡肽特异性测定法（高效液相色谱法与放射免疫分析法相结合），研究了电针（EA）对未处理、地塞米松（Dex）处理及肾上腺切除（Adrex）雄性SD大鼠的痛阈以及血浆、垂体（Pit）、下丘脑（Hyp）和脑脊液（CSF）中β-内啡肽（β-End）含量的影响。电针提高了痛阈和血浆β-内啡肽水平，对垂体、下丘脑和脑脊液中的β-内啡肽含量无影响。地塞米松不影响对照痛阈，但倾向于降低电针诱导的痛阈升高（电针镇痛，EAA）以及电针诱导的血浆β-内啡肽升高。肾上腺切除增加了血浆β-内啡肽水平，对照痛阈无变化。肾上腺切除倾向于降低电针镇痛效果，但在施加电针后观察到血浆β-内啡肽有进一步升高的趋势。肾上腺切除增加了垂体β-内啡肽水平，但施加电针后垂体β-内啡肽未观察到进一步变化。在未处理、地塞米松处理及肾上腺切除的大鼠中，观察到血浆β-内啡肽与血浆促肾上腺皮质激素之间呈正相关。在未处理、地塞米松处理及肾上腺切除的大鼠中，未观察到血浆β-内啡肽与电针镇痛效果之间的相关性。无电针刺激的后爪压力试验使血浆β-内啡肽升高的程度与电针刺激相同，但未产生镇痛作用。这些结果表明，垂体β-内啡肽可能不是电针镇痛作用产生的主要因素。
