Massabayeva Meruyert R, Aukenov N E, Mussazhanova Zh B, Saenko V A, Rogounovitch T I, Shaimardanov N K, Kurmanova B R, Barkibaeva N R, Rakhypbekov T K
Vopr Virusol. 2016;61(5):212-9.
This work is the first genetic association study of a potential relationship of single nucleotide polymorphisms rs8193036 and rs2275913 located in the IL17A promoter on chromosome 6p12 to chronic viral hepatitis and its progression in Kazakh population.
Evaluation of the effect of IL17A polymorphism on predisposition for chronic hepatitis B and C and its progression to liver cirrhosis.
A total of 862 individuals were enrolled in the retrospective case-control association study. Among the participants, 100 patients had chronic hepatitis B and/or C and liver cirrhosis, and 341 patients had chronic viral hepatitis only. Four hundred twenty-one (421) healthy HBV- and HCV-negative donors without liver diseases were recruited as population control. single nucleotide polymorphisms rs8193036[T/C] and rs2275913[G/A] were genotyped by TaqMan assays using genomic DNA extracted from peripheral blood cells. Results. Minor allele frequencies of rs8193036[C] and rs2275913[A] in the groups of patients were very similar to those observed in the control population, 0.4 and 0.3, respectively. Multivariate logistic regression analysis revealed odds ratios close to 1.0 and confidence intervals overlapping with the value of 1.0 and statistical significance p > 0.4 for any groups under comparison in the multiplicative model of inheritance.
No significant association between two single nucleotide polymorphisms, rs8193036 and rs2275913 in the IL17A promoter, and susceptibility to chronic viral hepatitis C and/or B and disease progression to liver cirrhosis in Kazakh population were found.
本研究首次对位于6号染色体p12区IL17A启动子上的单核苷酸多态性rs8193036和rs2275913与哈萨克族人群慢性病毒性肝炎及其进展之间的潜在关系进行基因关联研究。
评估IL17A基因多态性对慢性乙型和丙型肝炎易感性及其向肝硬化进展的影响。
共有862名个体纳入回顾性病例对照关联研究。参与者中，100例患有慢性乙型和/或丙型肝炎及肝硬化，341例仅患有慢性病毒性肝炎。招募421名无肝脏疾病的健康HBV和HCV阴性供者作为人群对照。采用TaqMan分析法对从外周血细胞中提取的基因组DNA进行单核苷酸多态性rs8193036[T/C]和rs2275913[G/A]基因分型。结果。患者组中rs8193036[C]和rs2275913[A]的次要等位基因频率与对照组人群中观察到的频率非常相似，分别为0.4和0.3。多因素逻辑回归分析显示，在遗传乘法模型中，任何比较组的优势比均接近1.0，置信区间与1.0值重叠，统计显著性p>0.4。
未发现IL17A启动子中的两个单核苷酸多态性rs8193036和rs2275913与哈萨克族人群慢性丙型和/或乙型病毒性肝炎易感性及疾病向肝硬化进展之间存在显著关联。
