Department of Cellular Biology, University of Georgia, Athens, Georgia 30602; email:
Annu Rev Entomol. 2018 Jan 7;63:1-13. doi: 10.1146/annurev-ento-020117-043447.
This article presents an overview of the development of techniques for analyzing cuticular proteins (CPs), their transcripts, and their genes over the past 50 years based primarily on experience in the laboratory of J.H. Willis. It emphasizes changes in the kind of data that can be gathered and how such data provided insights into the molecular underpinnings of insect metamorphosis and cuticle structure. It describes the techniques that allowed visualization of the location of CPs at both the anatomical and intracuticular levels and measurement of the appearance and deployment of transcripts from CP genes as well as what was learned from genomic and transcriptomic data. Most of the early work was done with the cecropia silkmoth, Hyalophora cecropia, and later work was with Anopheles gambiae.
本文主要基于 J.H.威利斯实验室的经验，概述了过去 50 年来分析表皮蛋白 (CPs)、其转录物和基因的技术的发展。它强调了可以收集的数据类型的变化，以及这些数据如何深入了解昆虫变态和表皮结构的分子基础。它描述了允许在解剖和细胞内水平上可视化 CPs 位置以及测量 CP 基因转录本的出现和部署的技术，以及从基因组和转录组数据中学到的内容。早期的大部分工作都是用秋粘虫，大造蚕蛾（Hyalophora cecropia）进行的，后来的工作是用冈比亚按蚊（Anopheles gambiae）进行的。
