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大肠杆菌K-12的htpR(rpoH)基因中温度敏感突变的鉴定。

Identification of a temperature-sensitive mutation in the htpR (rpoH) gene of Escherichia coli K-12.

作者信息

Waghorne C, Fuerst C R

出版信息

J Bacteriol. 1985 Nov;164(2):960-3. doi: 10.1128/jb.164.2.960-963.1985.

DOI:10.1128/jb.164.2.960-963.1985
PMID:2932429
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC214352/
Abstract

A new mutation in the htpR (rpoH) gene of Escherichia coli K-12 was identified. The mutation resulted in a temperature-sensitive phenotype in terms of cell growth and bacteriophage lambda development. As in the case of the classical htpR tsn-165 mutation, synthesis of heat shock polypeptides was not induced in strains carrying the mutation described here.

摘要

在大肠杆菌K-12的htpR(rpoH)基因中鉴定出一种新突变。就细胞生长和噬菌体λ发育而言,该突变导致温度敏感表型。与经典的htpR tsn-165突变情况一样,携带此处所述突变的菌株中未诱导热休克多肽的合成。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7fb0/214352/53a3a09137f4/jbacter00216-0478-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7fb0/214352/0970f924fa21/jbacter00216-0477-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7fb0/214352/53a3a09137f4/jbacter00216-0478-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7fb0/214352/0970f924fa21/jbacter00216-0477-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7fb0/214352/53a3a09137f4/jbacter00216-0478-a.jpg

相似文献

1
Identification of a temperature-sensitive mutation in the htpR (rpoH) gene of Escherichia coli K-12.大肠杆菌K-12的htpR(rpoH)基因中温度敏感突变的鉴定。
J Bacteriol. 1985 Nov;164(2):960-3. doi: 10.1128/jb.164.2.960-963.1985.
2
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Mutations in the rpoH (htpR) gene of Escherichia coli K-12 phenotypically suppress a temperature-sensitive mutant defective in the sigma 70 subunit of RNA polymerase.大肠杆菌K-12的rpoH(htpR)基因突变在表型上抑制了一个对RNA聚合酶σ70亚基有缺陷的温度敏感突变体。
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引用本文的文献

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A sigma32 mutant with a single amino acid change in the highly conserved region 2.2 exhibits reduced core RNA polymerase affinity.在高度保守的2.2区域发生单个氨基酸变化的sigma32突变体表现出核心RNA聚合酶亲和力降低。
Proc Natl Acad Sci U S A. 1997 May 13;94(10):4907-12. doi: 10.1073/pnas.94.10.4907.

本文引用的文献

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ISOLATION OF THE lambda PHAGE REPRESSOR.λ噬菌体阻遏物的分离
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Sensitive mutants of bacteriophage lambda.噬菌体λ的敏感突变体
Virology. 1961 May;14:22-32. doi: 10.1016/0042-6822(61)90128-3.
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The htpR gene product of E. coli is a sigma factor for heat-shock promoters.大肠杆菌的htpR基因产物是热休克启动子的一种σ因子。
Cell. 1984 Sep;38(2):383-90. doi: 10.1016/0092-8674(84)90493-8.
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Molecular cloning and expression of a gene that controls the high-temperature regulon of Escherichia coli.控制大肠杆菌高温调节子的一个基因的分子克隆与表达
J Bacteriol. 1983 Feb;153(2):597-603. doi: 10.1128/jb.153.2.597-603.1983.
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DNA packaging in the lambdoid phages: identification of the products of phi 80 genes 1 and 2.λ样噬菌体中的DNA包装:φ80基因1和2产物的鉴定
Virology. 1981 Jun;111(2):629-41. doi: 10.1016/0042-6822(81)90362-7.
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Interactions of bacteriophage and host macromolecules in the growth of bacteriophage lambda.噬菌体λ生长过程中噬菌体与宿主大分子的相互作用
Microbiol Rev. 1984 Dec;48(4):299-325. doi: 10.1128/mr.48.4.299-325.1984.
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Isolation and physical mapping of temperature-sensitive mutants defective in heat-shock induction of proteins in Escherichia coli.大肠杆菌中热休克诱导蛋白缺陷的温度敏感突变体的分离与物理图谱分析。
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