Department of Oral Medicine, Diagnosis and Periodontology, Faculty of Dentistry, Al Azhar University (Girls Branch), Cairo, Egypt.
Department of Oral Medicine, Diagnosis and Periodontology, Faculty of Dentistry, Nahda University, Beni-Suef, Egypt.
J Oral Pathol Med. 2018 Mar;47(3):286-292. doi: 10.1111/jop.12679. Epub 2018 Feb 5.
Although several studies have discussed some of the molecular and cellular changes associated with hereditary gingival fibromatosis (HGF), its pathogenesis is still largely unclear. This study was directed to detect and outline the degree of relationship between the immunophenotyped macrophages (M2) expressing CD163 and TGF-β1 in patients with gingival overgrowth due to HGF.
Biopsies from 20 patients suffering from HGF and 20 normal control subjects were harvested, histologically and immunohistochemically stained then, analyzed and statistically compared and correlated for CD163 immunoexpression and TGF-β1.
All HGF specimens expressed TGF-β1 by most of the connective tissue fibroblasts, with statistically high significant mean of area % (2.61 ± 0.41) compared to normal controls (0.11 ± 0.06; P = .001). All control specimens revealed negligible CD163 immunostaining of the few inflammatory cells found with a mean area of % (0.69 ± 0.12), while the specimens of HGF cases showed statistically significant higher CD163 expression (3.39 ± 0.75) at (P = .007). A statistically significant higher mean % of M2 cells expressing CD163 in relation to the total number of the inflammatory cells was revealed in HGF (34.46 ± 2.04) compared to the control group (16.36 ± 2.39; P-value ≤ .05). Moderate correlation between CD163 and TGF-β1 was detected in HGF (r = .451; P-value < .05).
CD163 and TGF-β1 were clearly expressed in HGF cases compared to healthy control patients, with significant correlation. In HGF, the increase in CD 163-positive cells was specific and not dependent on the chronic gingival inflammation.
虽然已有几项研究讨论了与遗传性牙龈纤维瘤病（HGF）相关的一些分子和细胞变化，但该病的发病机制仍很大程度上不清楚。本研究旨在检测和概述表达 CD163 的免疫表型巨噬细胞（M2）与 TGF-β1 在因 HGF 导致的牙龈过度生长患者中的关系程度。
采集 20 例 HGF 患者和 20 例正常对照者的活检组织，进行组织学和免疫组织化学染色，然后进行分析和统计学比较，并对 CD163 免疫表达和 TGF-β1 进行相关性分析。
所有 HGF 标本中的大多数结缔组织成纤维细胞均表达 TGF-β1，其面积百分比的均数（2.61±0.41）与正常对照组（0.11±0.06；P=0.001）相比具有统计学显著差异。所有对照标本中仅发现少数炎症细胞有可忽略不计的 CD163 免疫染色，其面积百分比的均数为（0.69±0.12），而 HGF 病例标本则显示出统计学上显著更高的 CD163 表达（3.39±0.75；P=0.007）。与炎症细胞总数相比，HGF 中表达 CD163 的 M2 细胞的平均百分比（34.46±2.04）明显更高，与对照组（16.36±2.39；P 值≤0.05）相比具有统计学显著差异。在 HGF 中检测到 CD163 与 TGF-β1 之间存在中度相关性（r=0.451；P 值<0.05）。
与健康对照组患者相比，HGF 病例中 CD163 和 TGF-β1 表达明显增加，且二者之间存在显著相关性。在 HGF 中，CD163 阳性细胞的增加是特异性的，与慢性牙龈炎症无关。
