Department of Aqualife Medicine, Chonnam National University, Republic of Korea.
Department of Marine Life Sciences, Jeju National University, Republic of Korea.
Vaccine. 2018 Feb 1;36(6):802-810. doi: 10.1016/j.vaccine.2017.12.077. Epub 2018 Jan 8.
Rock bream iridovirus (RBIV) causes severe mass mortalities in rock bream (Oplegnathus fasciatus) in Korea. In this study, we investigated the potential of viral membrane protein to induce antiviral status protecting rock bream against RBIV infection. We found that fish administered with ORF008L (myristoylated membrane protein, MMP) vaccine exhibited significantly higher levels of survival compared to ORF007L (major capsid protein, MCP). Moreover, ORF008L-based DNA vaccinated fish showed significant protection at 4 and 8 weeks post vaccination (wpv) than non-vaccinated fish after infected with RBIV (6.7 × 10) at 23 °C, with relative percent survival (RPS) of 73.36% and 46.72%, respectively. All of the survivors from the first RBIV infection were strongly protected (100% RPS) from re-infected with RBIV (1.1 × 10) at 100 dpi. In addition, the MMP (ORF008L)-based DNA vaccine significantly induced the gene expression of TLR3 (14.2-fold), MyD88 (11.6-fold), Mx (84.7-fold), ISG15 (8.7-fold), PKR (25.6-fold), MHC class I (13.3-fold), Fas (6.7-fold), Fas ligand (6.7-fold), caspase9 (17.0-fold) and caspase3 (15.3-fold) at 7 days post vaccination in the muscle (vaccine injection site). Our results showed the induction of immune responses and suggest the possibility of developing preventive measures against RBIV using myristoylated membrane protein-based DNA vaccine.
石斑鱼虹彩病毒（RBIV）在韩国导致石斑鱼（Oplegnathus fasciatus）大量死亡。在这项研究中，我们研究了病毒膜蛋白诱导抗病毒状态的潜力，以保护石斑鱼免受 RBIV 感染。我们发现，与 ORF007L（主要衣壳蛋白，MCP）疫苗相比，用 ORF008L（豆蔻酰化膜蛋白，MMP）疫苗接种的鱼的存活率明显更高。此外，在 23°C 下用 6.7×10 的 RBIV 感染后，与未接种疫苗的鱼相比，基于 ORF008L 的 DNA 疫苗接种鱼在 4 和 8 周攻毒后表现出显著的保护作用，相对存活率（RPS）分别为 73.36%和 46.72%。第一次 RBIV 感染后的所有幸存者均受到强烈保护（100%RPS），免受 100dpi 时再次感染 RBIV（1.1×10）。此外，MMP（ORF008L）基 DNA 疫苗在肌肉（疫苗注射部位）中显著诱导 TLR3（14.2 倍）、MyD88（11.6 倍）、Mx（84.7 倍）、ISG15（8.7 倍）、PKR（25.6 倍）、MHC 类 I（13.3 倍）、Fas（6.7 倍）、Fas 配体（6.7 倍）、caspase9（17.0 倍）和 caspase3（15.3 倍）的基因表达。我们的结果表明了免疫反应的诱导，并提出了使用豆蔻酰化膜蛋白基 DNA 疫苗预防 RBIV 的可能性。
