Antitoxin activity of aqueous extract of root against venom.

OBJECTIVES

Snakebites are a significant and severe global health problem. Till date, anti-snake venom serum is the only beneficial remedy existing on treating the snakebite victims. As antivenom was reported to induce early or late adverse reactions to human beings, snake venom neutralizing potential for root extract was tested for the present research by and approaches on toxin.

MATERIALS AND METHODS

evaluation of venom toxicity and neutralization assays was carried out. The root extracts from were used to evaluate the neutralization tests such as acetylcholinesterase, protease, direct hemolysis assay, phospholipase activity, and procoagulant activity. Gas chromatography-mass spectrometry (GC-MS) analysis from root extracts of was done to investigate the bioactive compounds.

RESULTS

The calculation of venom toxicity (LD) of venom remained to be 0.301 μg. root extracts were efficiently deactivated the venom lethality, and effective dose (ED) remained to be 7.24 mg/3LD of venom. root extract was found effective in counteracting all the lethal effects of venom. GC-MS analysis of the plant extract revealed the presence of antivenom compounds such as tetradecanoic and octadecadienoic acid which have neutralizing properties on venom.

CONCLUSION

The result from the and analysis indicates that plant root extract possesses significant compounds such as tetradecanoic acid hexadecanoic acid, heptadecanoic acid, and octadecadienoic acid which can counteract the toxins present in .