USDA, ARS, Foreign Disease Weed Science Research Unit, Fort Detrick, MD, USA.
AIDS and Cancer Virus Program, Leidos Biomedical Research Inc., Frederick National Laboratory for Cancer Research, Frederick, MD, USA.
Proteomics. 2018 Feb;18(3-4). doi: 10.1002/pmic.201700350. Epub 2018 Feb 2.
Rathayibacter toxicus is a Gram-positive bacterium that is the causative agent of annual ryegrass toxicity (ARGT), a disease that causes devastating losses in the Australian livestock industry. R. toxicus exhibits a complex life cycle, using the nematode Anguina funesta as a physical vector to carry it up to the seed head of the host plant. ARGT is caused by a tunicamycin-like corynetoxin that is produced in R. toxicus-infected seed galls. We analyzed protein expression in R. toxicus under stationary growth phase conditions to obtain a more complete understanding of the biology of this organism and identify potential targets for immunoassay development. A total of 323 unique proteins were identified, including those with putative roles in secondary metabolism and pathogenicity. The proteome analysis for this complex phytopathogenic Gram-positive bacterium will facilitate in the characterization of proteins necessary for host colonization and toxin production, and assist in the development of diagnostic assays. Data are available via ProteomeXchange with identifier PXD004238.
毒草菌是一种革兰氏阳性菌，是黑麦草毒性（ARGT）的病原体，这种疾病给澳大利亚畜牧业造成了毁灭性的损失。毒草菌表现出复杂的生命周期，利用线虫 Anguina funesta 作为物理载体将其携带到宿主植物的种子头部。ARGT 是由在毒草菌感染的种子瘤中产生的类似衣霉素的棒曲霉素引起的。我们分析了静止生长阶段毒草菌中的蛋白质表达，以更全面地了解该生物体的生物学特性，并确定免疫分析开发的潜在目标。共鉴定出 323 种独特的蛋白质，包括那些具有推测的次生代谢和致病性作用的蛋白质。对这种复杂的植物病原性革兰氏阳性菌的蛋白质组分析将有助于表征宿主定植和毒素产生所必需的蛋白质，并有助于开发诊断检测方法。数据可通过 ProteomeXchange 以标识符 PXD004238 获取。
