Bharathi D, Siddlingeshwar B, Thomas Anup, Kirilova Elena M, Nikolajeva Irena
Department of Physics, Ramaiah Institute of Technology, Bengaluru, India.
Centre for Computational Research in Clean Energy Technologies, Sree Chitra Thirunal College of Engineering, Trivandrum, India.
Luminescence. 2018 May;33(3):528-537. doi: 10.1002/bio.3442. Epub 2018 Jan 12.
The change in photophysical properties of the organic molecule due to solvatochromic effect caused by different solvent environments at room temperature gives information about the dipole moments of 3-N-(N'-methylacetamidino)benzanthrone (3-MAB). The quantum yield, fluorescence lifetime of 3-MAB was measured in different solvents to calculate radiative and non-radiative rate constants. The results revealed that the excited state dipole moment (μ ) is relatively larger compared to the ground state dipole moment (μ ), indicating the excited state of the dye under study is more polar than the ground state and the same trend is noticed with theoretical calculations performed using the CAM-B3LYP/6-311+G(d,p) method. Further, the study on preferential solvation was carried out for 3-MAB dye in ethyl acetate-methanol solvent mixture. The fluorescence quenching method has been employed for the detection of dopamine using 3-MAB as fluorescent probe, using steady-state and time resolved methods at room temperature. The method enables dopamine in the micro molar range to be detected. Also, an attempt to verify the quenching process by employing different models has been tried. Various rate parameters are measured using these models, our results indicates the quenching process is diffusion limited.
室温下不同溶剂环境引起的溶剂化显色效应导致有机分子光物理性质的变化，这提供了有关3-N-（N'-甲基乙酰脒基）苯并蒽酮（3-MAB）偶极矩的信息。在不同溶剂中测量了3-MAB的量子产率、荧光寿命，以计算辐射和非辐射速率常数。结果表明，激发态偶极矩（μ ）相对于基态偶极矩（μ ）相对较大，这表明所研究染料的激发态比基态更具极性，并且使用CAM-B3LYP/6-311+G(d,p)方法进行的理论计算也观察到了相同的趋势。此外，对3-MAB染料在乙酸乙酯 - 甲醇溶剂混合物中进行了优先溶剂化研究。采用荧光猝灭法，以3-MAB为荧光探针，在室温下使用稳态和时间分辨方法检测多巴胺。该方法能够检测微摩尔范围内的多巴胺。此外，还尝试采用不同模型验证猝灭过程。使用这些模型测量了各种速率参数，我们的结果表明猝灭过程受扩散限制。
