Department of Orthopedics, The Second Hospital of Shandong University, Shandong University, NO 247, Beiyuan Street, Jinan 250000, China.
Department of Orthopedics, Yidu Central Hospital of Weifang, South Linglongshan Road, NO 4138, Weifang 262500, China.
Future Oncol. 2018 Feb;14(5):461-469. doi: 10.2217/fon-2017-0490. Epub 2018 Jan 12.
The aim of this study is to explore the function of miR-20a in osteosarcoma.
MATERIALS & METHODS: miR-20a expression was measured by real-time PCR. miR-20a mimics, inhibitor and scramble siRNA were transfected into osteosarcoma cells to observe effects on colony formation and tumor growth. Moreover, relationships of miR-20a with TAK1 were investigated by western blot and luciferase activity.
We found that miR-20a was downregulated in osteosarcoma, and overexpression of miR-20a reduced colony formation and tumor growth. Furthermore, the data revealed that the function of miR-20a was probably exerted via targeting the TAK1 expression. Overexpression of miR-20a sensitizes the osteosarcoma cells to chemotherapeutic drugs.
Our data identify the role of miR-20a in osteosarcoma growth, indicating its potential application in chemotherapy.
本研究旨在探讨 miR-20a 在骨肉瘤中的功能。
实时 PCR 检测 miR-20a 的表达。将 miR-20a 模拟物、抑制剂和 scramble siRNA 转染到骨肉瘤细胞中，观察对集落形成和肿瘤生长的影响。此外，通过 Western blot 和荧光素酶活性检测 miR-20a 与 TAK1 的关系。
我们发现 miR-20a 在骨肉瘤中下调，过表达 miR-20a 可降低集落形成和肿瘤生长。此外，数据表明 miR-20a 的功能可能是通过靶向 TAK1 的表达来发挥的。过表达 miR-20a 可使骨肉瘤细胞对化疗药物敏感。
本研究鉴定了 miR-20a 在骨肉瘤生长中的作用，表明其在化疗中的潜在应用。
