1 Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University , Beijing, China .
2 Beijing Advanced Innovation Center for Biomedical Engineering, Beihang University , Beijing, China .
Tissue Eng Part B Rev. 2018 Aug;24(4):267-278. doi: 10.1089/ten.TEB.2017.0415. Epub 2018 Jul 13.
Induced pluripotent stem cells (iPSCs) hold enormous potential as a tool to generate cells for tissue engineering and regenerative medicine. Since the initial report of iPSCs in 2006, many different methods have been developed to enhance the safety and efficiency of this technology. Recent studies indicate that the extracellular signals can promote the production of iPSCs, and even replace the Yamanaka factors. Noticeably, abundant evidences suggest that the insoluble microenvironment, including the culture substrate and neighboring cells, directly regulates the expression of core pluripotency genes and the epigenetic modification of the chromatins, hence, impacts the reprogramming dynamics. These studies provide new strategies for developing safer and more efficient method for iPSC generation. In this review, we examine the publications addressing the insoluble extracellular microenvironment that boosts iPSC generation and self-renewal. We also discuss cell adhesion-mediated molecular mechanisms, through which the insoluble extracellular cues interplay with reprogramming.
诱导多能干细胞（iPSCs）作为组织工程和再生医学中生成细胞的工具具有巨大的潜力。自 2006 年首次报道 iPSCs 以来，已经开发出许多不同的方法来提高这项技术的安全性和效率。最近的研究表明，细胞外信号可以促进 iPSCs 的产生，甚至可以替代山中因子。值得注意的是，大量证据表明，包括培养底物和相邻细胞在内的不溶性微环境直接调节核心多能性基因的表达和染色质的表观遗传修饰，从而影响重编程动力学。这些研究为开发更安全、更有效的 iPSC 生成方法提供了新的策略。在这篇综述中，我们检查了涉及促进 iPSC 生成和自我更新的不溶性细胞外微环境的出版物。我们还讨论了细胞黏附介导的分子机制，通过该机制，不溶性细胞外信号与重编程相互作用。
