Evaluation of the use of recombinant human basic fibroblast growth factor in combination with negative pressure wound therapy with instillation and dwell time in porcine full-thickness wound model.

NPWT with instillation and dwell time (NPWTi-d), which combines NPWT with wound irrigation, has been clinically applied as a more effective treatment than conventional NPWT. Commercially available recombinant human basic fibroblast growth factor (rh-bFGF) has been demonstrated to be beneficial for use over the wound beds. The objective of this study was to evaluate the effectiveness of combined treatment with NPWTi-d and rh-bFGF. Six pigs received 12 full-thickness excisional skin wounds and were treated with six different treatment groups for each pair. The treatment regimens were composed NPWTi-d, NPWT, or advanced wound care with or without rh-bFGF. On day 6, the minimum granulation tissue thickness and blood vessel number of the group of combined treatment with NPWTi-d and rh-bFGF spray were significantly greater than that of the control group. Combined treatment with NPWTi-d and rh-bFGF spray reads to good granulation tissue formation and vascularization for accelerating wound healing.