Sentkowska Aleksandra, Pyrzynska Krystyna
University of Warsaw, Heavy Ion Laboratory, Pasteura 5A, 02-093 Warsaw, Poland.
University of Warsaw, Department of Chemistry, Pasteura 1, 02-093 Warsaw, Poland.
J Chromatogr B Analyt Technol Biomed Life Sci. 2018 Feb 1;1074-1075:8-15. doi: 10.1016/j.jchromb.2018.01.006. Epub 2018 Jan 8.
The hydrophilic interaction liquid chromatography (HILIC) coupled to mass spectrometry was employed to study retention behavior of selected selenium compounds using two different HILIC stationary phases: silica and zwitterionic. Two organic solvents - acetonitrile and methanol - were compared as a component of mobile phase. Separation parameters such as a content of organic modifier, the eluent pH and inorganic buffer concentration were investigated. Based on all observations, methanol seems to be beneficial for the separation of studied compounds. The optimal HILIC separation method involved silica column and eluent composed of 85% MeOH and CHCOONH (8 mM, pH 7) was compared to RP method in terms of time of the single run, the separation efficiency and limit of detection.
采用亲水作用液相色谱（HILIC）与质谱联用技术，使用两种不同的HILIC固定相：硅胶和两性离子固定相，研究了选定硒化合物的保留行为。比较了两种有机溶剂——乙腈和甲醇——作为流动相的组成成分。研究了有机改性剂含量、洗脱液pH值和无机缓冲液浓度等分离参数。基于所有观察结果，甲醇似乎有利于所研究化合物的分离。将最佳的HILIC分离方法（使用硅胶柱和由85%甲醇和醋酸铵（8 mM，pH 7）组成的洗脱液）与反相（RP）方法在单次运行时间、分离效率和检测限方面进行了比较。
