Department of Obstetrics and Gynecology, the Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou 325027, China; Department of Biomedical Science, Mercer University School of Medicine, Savannah, GA 31404, USA; Department of Obstetrics and Gynecology, Memorial Health Hospital, Mercer University School of Medicine, Savannah, GA 31404, USA.
Department of Obstetrics, Chongqing Health Center for Women and Children, Chongqing 400013, China.
Clin Chim Acta. 2019 Aug;495:637-645. doi: 10.1016/j.cca.2018.01.012. Epub 2018 Jan 10.
Cell fusion is a hallmark of placental trophoblast cell differentiation and the mature syncytiotrophoblasts play essential roles for fetal-maternal exchange and production of pregnancy-related hormones. Using a well-established in vitro trophoblast differentiation model, we performed a microarray analysis on mRNA expression in trophoblast and syncytiotrophoblast cell cultures. Dramatic changes in gene expression patterns were detected during trophoblast differentiation. Real-time PCR analysis confirmed the reliability of the microarray data. As many as 3524 novel and known genes have been found to be up- or down-regulated for >2-fold. A number of cell cycle regulator including CDC6, CDC20, Cyclins B2, L1 and E2, were down-regulated in the syncytiotrophoblast, providing a mechanism for the loss of mitotic activity during trophoblast differentiation. Further characterization on the identified genes may lead to better understanding of placental patho-physiology in obstetric diseases such as preeclampsia.
细胞融合是胎盘滋养层细胞分化的一个标志，成熟的合胞体滋养层在胎儿-母体交换和妊娠相关激素的产生中起着至关重要的作用。我们使用成熟的体外滋养层分化模型，对滋养层和合体滋养层细胞培养物中的 mRNA 表达进行了微阵列分析。在滋养层分化过程中，检测到基因表达模式的显著变化。实时 PCR 分析证实了微阵列数据的可靠性。多达 3524 个新的和已知的基因被发现上调或下调超过 2 倍。一些细胞周期调节剂，包括 CDC6、CDC20、细胞周期蛋白 B2、L1 和 E2，在合胞体滋养层中下调，为滋养层分化过程中细胞有丝分裂活性的丧失提供了一种机制。对鉴定基因的进一步特征分析可能有助于更好地理解子痫前期等产科疾病中的胎盘病理生理学。
