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兔肝磷酸葡萄糖变位酶的作用机制。

Mechanism of action of rabbit liver phosphoglucomutase.

作者信息

Jamil H, Clarke J B

出版信息

Biochem J. 1985 Sep 15;230(3):791-5. doi: 10.1042/bj2300791.

Abstract

Induced-transport tests with comparatively undegraded rabbit liver phosphoglucomutase show that the enzyme possesses a phosphoenzyme mechanism and that any interconversion of phosphoenzyme forms is very rapid. A relatively stable 32P-labelled phosphoenzyme was isolated, which exchanged label rapidly with substrates. The phospho group appears to be bonded to a serine residue on the enzyme.

摘要

用相对未降解的兔肝磷酸葡萄糖变位酶进行的诱导转运试验表明,该酶具有磷酸化酶机制,并且磷酸化酶形式的任何相互转化都非常迅速。分离出一种相对稳定的32P标记的磷酸化酶,它能与底物快速交换标记。磷酸基团似乎与酶上的一个丝氨酸残基结合。

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