Sterner D C, Zaks W J, Creutz C E
Biochem Biophys Res Commun. 1985 Oct 30;132(2):505-12. doi: 10.1016/0006-291x(85)91162-3.
The influence of fatty acids on the polymerization of synexin was studied by monitoring light scattered from solutions of purified synexin. Cis-unsaturated fatty acids such as arachidonate or oleate stimulated synexin polymerization at sub-micromolar concentrations, while saturated fatty acids, a trans unsaturated fatty acid or a fatty acid methyl ester had little effect. The polymerization of synexin occurred at lower concentrations of Ca2+ in the presence of the fatty acids than in the absence of fatty acids. Therefore, Ca2+ and free fatty acids may act as co-regulators of synexin action in stimulated secretory cells.
通过监测纯化的突触结合蛋白溶液散射的光，研究了脂肪酸对突触结合蛋白聚合的影响。顺式不饱和脂肪酸如花生四烯酸或油酸在亚微摩尔浓度下刺激突触结合蛋白聚合，而饱和脂肪酸、反式不饱和脂肪酸或脂肪酸甲酯几乎没有影响。与不存在脂肪酸的情况相比，在脂肪酸存在下，突触结合蛋白的聚合在较低浓度的Ca2+时发生。因此，Ca2+和游离脂肪酸可能作为受刺激的分泌细胞中突触结合蛋白作用的共同调节因子。
