Molecular Medicine Research Laboratories, Robarts Research Institute and Department of Microbiology & Immunology, University of Western Ontario, 1151 Richmond Street North, London, Ontario, N6A 5B7, Canada.
Imaging Research Laboratories, Robarts Research Institute and Department of Microbiology & Immunology, University of Western Ontario, 1151 Richmond Street North, London, Ontario, N6A 5B7, Canada.
Sci Rep. 2018 Jan 12;8(1):590. doi: 10.1038/s41598-017-19031-0.
A Fluorine (F) perfluorocarbon cell labeling agent, when employed with an appropriate cellular MRI protocol, allows for in vivo cell tracking. F cellular MRI can be used to non-invasively assess the location and persistence of cell-based cancer vaccines and other cell-based therapies. This study was designed to determine the feasibility of labeling and tracking peripheral blood mononuclear cells (PBMC), a heterogeneous cell population. Under GMP-compliant conditions human PBMC were labeled with a F-based MRI cell-labeling agent in a manner safe for autologous re-injection. Greater than 99% of PBMC labeled with the F cell-labeling agent without affecting functionality or affecting viability. The F-labeled PBMC were detected in vivo in a mouse model at the injection site and in a draining lymph node. A clinical cellular MR protocol was optimized for the detection of PBMC injected both at the surface of a porcine shank and at a depth of 1.2 cm, equivalent to depth of a human lymph node, using a dual H/F dual switchable surface radio frequency coil. This study demonstrates it is feasible to label and track F-labeled PBMC using clinical MRI protocols. Thus, F cellular MRI represents a non-invasive imaging technique suitable to assess the effectiveness of cell-based cancer vaccines.
一种氟（F）全氟碳细胞标记剂，与适当的细胞 MRI 方案一起使用时，可实现体内细胞跟踪。F 细胞 MRI 可用于非侵入性评估基于细胞的癌症疫苗和其他基于细胞的疗法的位置和持久性。本研究旨在确定标记和跟踪外周血单核细胞（PBMC）的可行性，这是一种异质细胞群体。在符合 GMP 标准的条件下，以适合自体回注的方式用 F 基 MRI 细胞标记剂标记人 PBMC。>99%的 PBMC 被 F 细胞标记剂标记，而不会影响功能或活力。F 标记的 PBMC 在注射部位和引流淋巴结的小鼠模型中可在体内检测到。使用双 H/F 双切换表面射频线圈，优化了临床细胞 MR 方案，以检测在猪胫骨表面和 1.2cm 深处（相当于人类淋巴结的深度）注射的 PBMC，等效于人类淋巴结的深度。该研究表明，使用临床 MRI 方案标记和跟踪 F 标记的 PBMC 是可行的。因此，F 细胞 MRI 代表了一种非侵入性成像技术，适合评估基于细胞的癌症疫苗的有效性。
