Skin Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Br J Dermatol. 2018 Jul;179(1):72-79. doi: 10.1111/bjd.16338. Epub 2018 May 10.
Different methods of fibroblast application have been examined to treat recessive dystrophic epidermolysis bullosa (RDEB).
To compare the effects of intradermal injection of cultured allogeneic fibroblasts in healing RDEB wounds with those of fibroblasts seeded on amniotic membrane scaffolds (FAMS) or standard wound care (SWC) with Vaseline gauze as controls.
Seven patients were recruited, and seven wounds were assessed in each patient: three wounds were treated with injection of intradermal fibroblasts, three were treated with FAMS and one was dressed with SWC. Changes in wound size were assessed after 2 and 12 weeks of treatment. Qualitative wound scores (QWS) were used to assess wound severity. Additionally, biopsies and antigen mapping were performed to detect type VII collagen in the dermoepidermal junction.
In both treated areas, the QWS and wound size were significantly decreased (P < 0·001), whereas there were no changes in the control group (P = 0·29). After 2 and 12 weeks of treatment, the wound size was significantly decreased in wounds that were treated with fibroblast injection compared with those treated with FAMS (P < 0·001); but no significant changes were found in the control group.
Fibroblast injection has been shown to promote healing of RDEB wounds and is superior to FAMS or the control treatment.
已经研究了不同的成纤维细胞应用方法来治疗隐性营养不良型大疱性表皮松解症（RDEB）。
比较真皮内注射培养的同种异体成纤维细胞与纤维母细胞接种于羊膜支架（FAMS）或凡士林纱布（SWC）标准伤口护理（SWC）治疗 RDEB 伤口的效果，以凡士林纱布作为对照。
招募了 7 名患者，每位患者评估了 7 个伤口：3 个伤口用真皮内注射成纤维细胞治疗，3 个伤口用 FAMS 治疗，1 个伤口用 SWC 治疗。治疗 2 周和 12 周后评估伤口大小的变化。使用定性伤口评分（QWS）评估伤口严重程度。此外，还进行了活检和抗原定位，以检测表皮连接部的 VII 型胶原蛋白。
在治疗区域，QWS 和伤口大小均显著降低（P<0.001），而对照组无变化（P=0.29）。治疗 2 周和 12 周后，与 FAMS 治疗相比，成纤维细胞注射治疗的伤口大小明显减小（P<0.001）；但对照组无明显变化。
成纤维细胞注射已被证明可促进 RDEB 伤口愈合，优于 FAMS 或对照治疗。
