College of Animal Sciences, Zhejiang University, Hangzhou, China; Department of Animal and Poultry Science, University of Saskatchewan, Saskatoon S7N 5A8, Canada.
College of Animal Sciences, Zhejiang University, Hangzhou, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2018 Apr 5;194:181-188. doi: 10.1016/j.saa.2018.01.019. Epub 2018 Jan 5.
Advanced vibrational molecular spectroscopy has been developed as a rapid and non-destructive tool to reveal intrinsic molecular structure conformation of biological tissues. However, this technique has not been used to systematically study flaking induced structure changes at a molecular level. The objective of this study was to use vibrational molecular spectroscopy to reveal association between steam flaking induced CHO molecular structural changes in relation to grain CHO fractionation, predicted CHO biodegradation and biodigestion in ruminant system. The Attenuate Total Reflectance Fourier-transform Vibrational Molecular Spectroscopy (ATR-Ft/VMS) at SRP Key Lab of Molecular Structure and Molecular Nutrition, Ministry of Agriculture Strategic Research Chair Program (SRP, University of Saskatchewan) was applied in this study. The fractionation, predicted biodegradation and biodigestion were evaluated using the Cornell Net Carbohydrate Protein System. The results show that: (1) The steam flaking induced significant changes in CHO subfractions, CHO biodegradation and biodigestion in ruminant system. There were significant differences between non-processed (raw) and steam flaked grain corn (P<.01); (2) The ATR-Ft/VMS molecular technique was able to detect the processing induced CHO molecular structure changes; (3) Induced CHO molecular structure spectral features are significantly correlated (P<.05) to CHO subfractions, CHO biodegradation and biodigestion and could be applied to potentially predict CHO biodegradation (R=0.87, RSD=0.74, P<.01) and intestinal digestible undegraded CHO (R=0.87, RSD=0.24, P<.01). In summary, the processing induced molecular CHO structure changes in grain corn could be revealed by the ATR-Ft/VMS vibrational molecular spectroscopy. These molecular structure changes in grain were potentially associated with CHO biodegradation and biodigestion.
先进的振动分子光谱学已经发展成为一种快速、非破坏性的工具，可以揭示生物组织的固有分子结构构象。然而，这项技术尚未用于系统地研究剥落诱导的分子水平结构变化。本研究的目的是使用振动分子光谱学揭示蒸汽剥落诱导 CHO 分子结构变化与谷物 CHO 分级、反刍动物系统中预测的 CHO 生物降解和生物消化之间的关系。本研究应用了加拿大萨斯喀彻温省农业部战略研究主席计划（SRP，萨斯喀彻温大学）SRP 关键实验室的衰减全反射傅里叶变换振动分子光谱（ATR-Ft/VMS）。采用康奈尔净碳水化合物-蛋白质体系评价分级、预测生物降解和生物消化。结果表明：（1）蒸汽剥落导致反刍动物系统中 CHO 亚组分、CHO 生物降解和生物消化发生显著变化。未加工（原）和蒸汽剥落谷物玉米之间存在显著差异（P<.01）；（2）ATR-Ft/VMS 分子技术能够检测到加工诱导的 CHO 分子结构变化；（3）诱导的 CHO 分子结构光谱特征与 CHO 亚组分、CHO 生物降解和生物消化显著相关（P<.05），并可用于潜在预测 CHO 生物降解（R=0.87，RSD=0.74，P<.01）和肠道可消化未降解 CHO（R=0.87，RSD=0.24，P<.01）。总之，谷物玉米中由加工引起的 CHO 分子结构变化可以通过 ATR-Ft/VMS 振动分子光谱学来揭示。这些谷物中的分子结构变化可能与 CHO 的生物降解和生物消化有关。
