SiHa宫颈癌细胞中miR-19a抑制与放射敏感性之间的相关性。
Correlation between miR-19a inhibition and radiosensitivity in SiHa cervical cancer cells.
作者信息
Wang Yina, Wang Yang, Zhong Wei, Gulina Kuerban
机构信息
First Department of Gynecological Tumor Radiotherapy, Affiliated Tumor Hospital of Xinjiang Medical University, Urumqi 830011, China.
出版信息
J BUON. 2017 Nov-Dec;22(6):1505-1508.
PURPOSE
MicroRNAs (miRs) have been implicated in many aspects of tumor cell development and survival, including sensitivity to radiotherapy. In particular, miR-19a regulates the proliferation of cervical cancer cells, but its role in radiosensitivity is not known. Here, we describe the consequence of silencing miR-19a using antisense oligonucleotides (ASO) on the radiosensitivity of human cervical cancer SiHa cells.
METHODS
Following transfection with miR-19a-ASO or control-ASO, SiHa cells were exposed to X-rays to determine their proliferation.
RESULTS
Silencing of miR-19a significantly improved the sensitivity of SiHa cells to radiotherapy by reducing proliferation, increasing apoptosis, upregulating BAX, and downregulating Bcl-2.
CONCLUSION
Overall, inhibiting miR-19a significantly improves the sensitivity of SiHa cells to radiotherapy, which could lead to new methods for the treatment of cervical cancer.
目的
微小RNA（miR）已被证明在肿瘤细胞发育和存活的许多方面发挥作用，包括对放疗的敏感性。特别是，miR-19a调节宫颈癌细胞的增殖，但其在放射敏感性中的作用尚不清楚。在此，我们描述了使用反义寡核苷酸（ASO）沉默miR-19a对人宫颈癌SiHa细胞放射敏感性的影响。
方法
用miR-19a-ASO或对照ASO转染后，将SiHa细胞暴露于X射线下以测定其增殖情况。
结果
沉默miR-19a可通过减少增殖、增加凋亡、上调BAX和下调Bcl-2来显著提高SiHa细胞对放疗的敏感性。
结论
总体而言，抑制miR-19a可显著提高SiHa细胞对放疗的敏感性，这可能为宫颈癌治疗带来新方法。
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