Department of Analytical Chemistry, Physical Chemistry and Chemical Engineering, University of Alcalá, E-28871 Alcala de Henares, Madrid, Spain.
Department of Analytical Chemistry, Physical Chemistry and Chemical Engineering, University of Alcalá, E-28871 Alcala de Henares, Madrid, Spain; Chemical Research Institute "Andrés M. del Río" (IQAR), University of Alcalá, E-28805 Alcalá de Henares, Madrid, Spain.
Talanta. 2018 Apr 1;180:206-210. doi: 10.1016/j.talanta.2017.12.018. Epub 2017 Dec 13.
Alpha-1-acid glycoprotein (AGP) or Orosomucoid is a serum glycoprotein which belongs to the group of acute phase proteins. It is a potential biomarker for inflammatory bowel diseases. In this sense, there is a need for developing fast and cheap analytical methods for diagnosis, prognosis and follow-up of these diseases. In this work, we propose a simple and cheap electrochemical method for total AGP determination using disposable carbon screen-printed electrodes (SPE) and using a selective acidic precipitation of the rest of proteins. This method avoids the use of biological components, decreasing dramatically the analysis cost. Firstly, AGP is labeled with an electrochemical tag (osmium (VI) complex) and then the total amount of AGP is quantified by adsorptive transfer stripping square wave voltammetry. The method optimized showed a good linear correlation (r = 0.9992) and limit of detection of 1.6mgl. The methodology was successfully applied to quantify AGP in a commercial serum sample. This methodology could be useful in clinical diagnosis because of AGP levels increase two or three times when inflammatory processes happen. Moreover, the inherent advantages of SPE technology (low sample consumption, low cost and point of care testing) make this methodology very attractive in this field.
α-1-酸性糖蛋白（AGP）或乳白蛋白是一种血清糖蛋白，属于急性期蛋白。它是炎症性肠病的潜在生物标志物。在这方面，需要开发快速、廉价的分析方法来诊断、预后和监测这些疾病。在这项工作中，我们提出了一种简单廉价的电化学方法，使用一次性碳丝网印刷电极（SPE）和选择性酸性沉淀其余蛋白质来测定总 AGP。该方法避免使用生物成分，大大降低了分析成本。首先，AGP 用电化学标签（锇（VI）配合物）标记，然后通过吸附转移方波伏安法定量测定总 AGP 量。优化后的方法显示出良好的线性相关性（r = 0.9992）和检测限为 1.6mgl。该方法成功应用于商业血清样本中 AGP 的定量。由于炎症过程发生时 AGP 水平增加两到三倍，因此该方法在临床诊断中可能具有重要意义。此外，SPE 技术的固有优势（低样品消耗、低成本和即时检测）使该方法在该领域非常有吸引力。
