采用免疫捕获萃取和液相色谱-质谱联用技术对人尿液中的石房蛤毒素进行定量分析。
Quantitation of saxitoxin in human urine using immunocapture extraction and LC-MS.
作者信息
Bragg William A, Garrett Alaine, Hamelin Elizabeth I, Coleman Rebecca M, Campbell Katrina, Elliott Christopher T, Johnson Rudolph C
机构信息
Emergency Response Branch, Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control & Prevention, Atlanta, GA, 30341 USA.
ORISE Fellow, Centers for Disease Control & Prevention, National Center for Environmental Health, Division of Laboratory Sciences, Atlanta, GA 30341 USA.
出版信息
Bioanalysis. 2018 Feb;10(4):229-239. doi: 10.4155/bio-2017-0156. Epub 2018 Jan 15.
AIM
An immunomagnetic capture protocol for use with LC-MS was developed for the quantitation of saxitoxin (STX) in human urine.
MATERIALS & METHODS: This method uses monoclonal antibodies coupled to magnetic beads. STX was certified reference material grade from National Research Council, Canada. Analysis was carried out using LC-MS.
RESULTS
With an extraction efficiency of 80%, accuracy and precision of 93.0-100.2% and 5.3-12.6%, respectively, and a dynamic range of 1.00-100 ng/ml, the method is well suited to quantify STX exposures based on previously reported cases.
CONCLUSION
Compared with our previously published protocols, this method has improved selectivity, a fivefold increase in sensitivity and uses only a third of the sample volume. This method can diagnose future toxin exposures and may complement the shellfish monitoring programs worldwide.
目的
开发一种用于液相色谱 - 质谱联用（LC-MS）的免疫磁捕获方法，用于定量检测人尿液中的石房蛤毒素（STX）。
材料与方法
该方法使用与磁珠偶联的单克隆抗体。STX为加拿大国家研究委员会认证的参考物质级。采用LC-MS进行分析。
结果
该方法提取效率为80%，准确度为93.0 - 100.2%，精密度为5.3 - 12.6%，动态范围为1.00 - 100 ng/ml，非常适合根据先前报道的病例定量检测STX暴露情况。
结论
与我们之前发表的方法相比，该方法选择性更高，灵敏度提高了五倍，且仅使用三分之一的样品体积。该方法可诊断未来的毒素暴露情况，并可能补充全球范围内的贝类监测计划。
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