Morales-Estrada Aurea I, Lopez-Merino Ahide, Gutierrez-Mendez Nestor, Ruiz Enrico A, Contreras-Rodriguez Ararceli
Department of Microbiology, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Mexico City, Mexico.
Chemistry Faculty, Universidad Autónoma de Chihuahua, Chihuahua, Mexico.
Pol J Microbiol. 2016 Aug 26;65(3):279-285. doi: 10.5604/17331331.1215607.
During a screening of lactic acid bacteria producing bacteriocin from Cotija cheese, the strain QC38 was isolated. Based on the 16S rRNA gene nucleotide sequencing (516 pb accession no KJ210322) and phylogenetic analysis, the isolate was identified as Pediococcus acidilactici. Neutralized cell-free supernatant was tested for antimicrobial activity against 17 Gram-negative and Gram-positive pathogens. Growth inhibition was achieved against Listeria monocytogenes (supplier or indication or source), Staphylococcus aureus, Vibrio vulnificus, Vibrio cholerae O1 Ogawa, Vibrio cholerae NO 01 and Salmonella enterica subsp. Enterica serovar Typhimurium. Bacteriocin-like substance, after heating at 121°C for 15 min it remained stable and its antimicrobial activity was observed at pH ranging from 1.0 to 10.0 but inactivated by α-chymotrypsin and proteinase K. Strain QC38 was able to grow in 1-9% NaCl concentration. The plate overlay assay showed an approximate size of bacteriocin-like substance between 3.4 and 6.5 kDa. P. acidilactici QC38 harboured a plasmid that contains a gene for a pediocin (PA-1).
在对从科蒂亚奶酪中筛选产细菌素的乳酸菌进行的研究中，分离出了菌株QC38。基于16S rRNA基因核苷酸测序（516个碱基对，登录号KJ210322）和系统发育分析，该分离株被鉴定为嗜酸乳杆菌。对中和后的无细胞上清液针对17种革兰氏阴性和革兰氏阳性病原体的抗菌活性进行了测试。对单核细胞增生李斯特菌（供应商或指示或来源）、金黄色葡萄球菌、创伤弧菌、霍乱弧菌O1小川型、霍乱弧菌非O1型以及肠炎沙门氏菌肠炎亚种鼠伤寒血清型均实现了生长抑制。类细菌素物质在121°C加热15分钟后仍保持稳定，其抗菌活性在pH值为1.0至10.0的范围内均有观察到，但可被α-胰凝乳蛋白酶和蛋白酶K灭活。菌株QC38能够在1%-9%的NaCl浓度下生长。平板覆盖试验显示类细菌素物质的大小约在3.4至6.5 kDa之间。嗜酸乳杆菌QC38携带一个含有植物乳杆菌素（PA-1）基因的质粒。
