Mahdhi Mosbah, Houidheg Nadia, Mahmoudi Neji, Msaadek Abdelhakim, Rejili Mokhtar, Mars Mohamed
Center for Environmental Research and Studies, Jazan University, Jazan, Kingdom of Saudi Arabia.
Research Unit Biodiversity and Valorization of Arid Areas, Bioressources (BVBAA), Faculty of Sciences, Gabès University, Erriadh-Zrig, Gabès, Tunisia.
Pol J Microbiol. 2016 Aug 26;65(3):331-339. doi: 10.5604/17331331.1215612.
Fifty seven bacterial isolates from root nodules of two spontaneous legumes (Astragalus corrugatus and Hippocrepis areolata) growing in the arid areas of Tunisia were characterized by phenotypic features, 16S rDNA PCR-RFLP and 16S rRNA gene sequencing. Phenotypically, our results indicate that A. corrugatus and H. areolata isolates showed heterogenic responses to the different phenotypic features. All isolates were acid producers, fast growers and all of them used different compounds as sole carbon and nitrogen source. The majority of isolate grew at pHs between 6 and 9, at temperatures up to 40°C and tolerated 3% NaCl concentrations. Phylogenetically, the new isolates were affiliated to four genera Sinorhizobium, Rhizobium, Mesorhizobium and Agrobacterium. About 73% of the isolates were species within the genera Sinorhizobium and Rhizobium. The isolates which failed to nodulate their host plants of origin were associated to Agrobacterium genus (three isolates).
从突尼斯干旱地区生长的两种自生豆科植物（皱黄芪和网纹马蹄豆）的根瘤中分离出57株细菌，通过表型特征、16S rDNA PCR-RFLP和16S rRNA基因测序对其进行了鉴定。在表型上，我们的结果表明，皱黄芪和网纹马蹄豆的分离株对不同的表型特征表现出异质性反应。所有分离株均产酸，生长迅速，且都利用不同化合物作为唯一碳源和氮源。大多数分离株在pH值6至9、温度高达40°C的条件下生长，并耐受3%的NaCl浓度。在系统发育上，新分离株隶属于中华根瘤菌属、根瘤菌属、中慢生根瘤菌属和农杆菌属四个属。约73%的分离株属于中华根瘤菌属和根瘤菌属中的物种。未能使其原生寄主植物结瘤的分离株与农杆菌属有关（三株分离株）。
