Thammasittirong Sutticha Na-Ranong, Chamduang Thada, Phonrod Umaporn, Sriroth Klanarong
Department of Founding Project of Microbiology, Faculty of Liberal Arts and Science, Kasetsart University Kampheang Saen Campus, Nakorn Pathom, Thailand.
Department of Science, Faculty of Liberal Arts and Science, Kasetsart University Kamphaeng Sean Campus, Nakorn Pathom, Thailand.
Pol J Microbiol. 2012 Sep 28;61(3):219-221.
Four ethanologenic ethanol-tolerant yeast strains, Saccharomyces cerevisiae (ATKU132), Saccharomycodes ludwigii (ATKU47), and Issatchenkia orientalis (ATKU5-60 and ATKU5-70), were isolated by an enrichment technique in yeast extract peptone dextrose (YPD) medium supplemented with 10% (v/v) ethanol at 30°C. Among non-Saccharomyces yeasts, Sd. ludwigii ATKU47 exhibited the highest ethanol-tolerance and ethanol production, which was similar to S. cerevisiae ATKU132. The maximum range of ethanol concentrations produced at 37°C by S. cerevisiae ATKU132 and Sd. ludwigii ATKU47 from an initial D-glucose concentration of 20% (w/v) and 28% (w/v) sugarcane molasses were 9.46-9.82% (w/v) and 8.07-8.32% (w/v), respectively.
通过富集培养技术，在30°C下于添加了10%（v/v）乙醇的酵母提取物蛋白胨葡萄糖（YPD）培养基中，分离出了4株产乙醇且耐乙醇的酵母菌株，分别为酿酒酵母（ATKU132）、路德类酵母（ATKU47）和东方伊萨酵母（ATKU5 - 60和ATKU5 - 70）。在非酿酒酵母中，路德类酵母ATKU47表现出最高的乙醇耐受性和乙醇产量，这与酿酒酵母ATKU132相似。酿酒酵母ATKU132和路德类酵母ATKU47在37°C下，以初始浓度为20%（w/v）的D -葡萄糖以及28%（w/v）的甘蔗 molasses为原料，所产生的乙醇浓度最大范围分别为9.46 - 9.82%（w/v）和8.07 - 8.32%（w/v）。
