State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, Hunan University , Changsha, Hunan 410082, P. R. China.
Institute of Pharmacy and Pharmacology, University of South China , Hengyang, Hunan 421001, P. R. China.
Anal Chem. 2018 Feb 20;90(4):2501-2507. doi: 10.1021/acs.analchem.7b03636. Epub 2018 Jan 29.
Creating color difference and improving the color resolution in digital imaging is crucial for better application of color analysis. Herein, a novel color modulation analysis strategy was developed by using a homemade tunable multiband laser illumination device, in which the portions of R, G, and B components of the illumination light are discretionarily adjustable, and hence the sample color could be visually modulated continuously in the RGB color space. Through this strategy, the color appearance of single gold nanorods (AuNRs) under dark-field microscopy was migrated from the spectrally insensitive red region to the spectrally sensitive green-yellow region. Unlike the traditional continuous-wave light source illumination, wherein the small spectral variations in the samples within a narrow spectral range are averaged by the whole spectrum of the light source, leading to little color difference, the application of sharp, multiband laser illumination could enlarge the color separation between samples, thus resulting in high spectral sensitivity in color analysis. By comparing the corresponding color evolution processes of different samples as the multiband combination of the laser illumination was changed, more efficient color separation of AuNRs was achieved. With this instrument and single Ag@AuNRs as the sulfide probe, we achieved high throughput and highly sensitive detection of sulfide at a detection limit of 0.1 nM, a more than 2 orders of magnitude improvement compared to the previous color sensing scheme. This strategy could be utilized for nanoparticle identification, evaluation, and determination in biological imaging and biochemical analysis.
在数字成像中，创建色差并提高颜色分辨率对于更好地应用颜色分析至关重要。在此，我们通过使用自制的可调谐多波段激光照明设备，开发了一种新的颜色调制分析策略。通过该策略，暗场显微镜下单个金纳米棒（AuNRs）的颜色外观从光谱不敏感的红色区域迁移到光谱敏感的绿黄色区域。与传统的连续波光源照明不同，在连续波光源照明下，光源的整个光谱会平均样品在较窄光谱范围内的小光谱变化，导致颜色差异较小，而锐线多波段激光照明的应用可以扩大样品之间的颜色分离，从而在颜色分析中实现高光谱灵敏度。通过比较不同样品在激光照明多波段组合变化时的相应颜色演变过程，可以实现 AuNRs 的更高效颜色分离。使用该仪器和单个 Ag@AuNRs 作为硫化物探针，我们实现了硫化物的高通量和高灵敏度检测，检测限为 0.1 nM，比以前的颜色感应方案提高了 2 个数量级。该策略可用于生物成像和生化分析中的纳米颗粒识别、评估和测定。
