Goldmacher V S, Anderson J, Blättler W A, Lambert J M, Senter P D
J Immunol. 1985 Dec;135(6):3648-51.
The antibody-mediated cytotoxicity of complement can be increased by the ribosome-inactivating proteins gelonin and PAP-S. Treatment of human lymphoid cells that express CALLA with an anti-CALLA monoclonal antibody, J5, and then with rabbit complement, leaves about 6% of the cells alive. The same treatment in the presence of a sublethal concentration of gelonin or PAP-S leaves only about 0.02 and 0.3% of the cells alive respectively. This synergistic effect has potential implications for the in vitro elimination of malignant cells or of immunocompetent cells from bone marrow before its transplantation.
核糖体失活蛋白gelonin和PAP - S可增强补体的抗体介导细胞毒性。用抗CALLA单克隆抗体J5处理表达CALLA的人淋巴细胞，然后加入兔补体，约6%的细胞存活。在亚致死浓度的gelonin或PAP - S存在下进行相同处理，分别仅有约0.02%和0.3%的细胞存活。这种协同效应对于体外清除恶性细胞或在骨髓移植前从骨髓中清除免疫活性细胞具有潜在意义。
